Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Human immunodeficiency virus 1 |
Crystallization (Comment) | Organism |
---|---|
in complex with substrate, hanging drop vapor diffusion method, using 50 mM Bis-Tris propane-HCl pH 7.0, 10% PEG 8000 (w/v), 100 mM ammonium sulfate, 5% glycerol (v/v), 5% sucrose (w/v) and 20 mM MgCl2 | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
D498N | active site mutant | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | P03366 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and heparin column chromatography | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H | - |
Human immunodeficiency virus 1 |
RNase H | - |
Human immunodeficiency virus 1 |