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Literature summary for 3.1.26.13 extracted from

  • Biondi, M.J.; Beilhartz, G.L.; McCormick, S.; Goette, M.
    N348I in HIV-1 reverse transcriptase can counteract the nevirapine-mediated bias toward RNase H cleavage during plus-strand initiation (2010), J. Biol. Chem., 285, 26966-26975.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.13

Protein Variants

Protein Variants Comment Organism
E478Q RNase H active site mutant Human immunodeficiency virus 1
E478Q/N348I mutation of RNase H active site and connection domain Human immunodeficiency virus 1
K103N a classic NNRTI resistance mutation Human immunodeficiency virus 1
N348I connection domain mutant, altered RNase H cleavage pattern compared to the wild-type HIV-1 RT Human immunodeficiency virus 1
Y181C a classic NNRTI resistance mutation Human immunodeficiency virus 1

Inhibitors

Inhibitors Comment Organism Structure
efavirenz stronger effect of mutation N348I on RNase H susceptibility to nevirapine as compared with efavirenz Human immunodeficiency virus 1
additional information non-nucleoside RT inhibitors, NNRTIs, bind to an allosteric site that is 10 A from the polymerase active site and 60 A from the RNase H active site Human immunodeficiency virus 1
nevirapine a non-nucleoside RT inhibitor, NNRTI, stronger effect of mutation N348I on RNase H susceptibility to nevirapine as compared with efavirenz Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
 HIV-1
-
Human immunodeficiency virus 1 HXB-2
-
 HIV-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleavage of DNA-RNA and RNA-DNA primer templates Human immunodeficiency virus 1 ?
-
 ?
additional information cleavage of DNA-RNA and RNA-DNA primer templates Human immunodeficiency virus 1 HXB-2 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H
-
 Human immunodeficiency virus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human immunodeficiency virus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Human immunodeficiency virus 1