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Literature summary for 3.1.26.13 extracted from

  • Mizuno, M.; Yasukawa, K.; Inouye, K.
    Insight into the mechanism of the stabilization of Moloney murine leukaemia virus reverse transcriptase by eliminating RNase H activity (2010), Biosci. Biotechnol. Biochem., 74, 440-442.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Moloney murine leukemia virus

Protein Variants

Protein Variants Comment Organism
D524A mutant lacks RNase H activity, but retains reverse transcriptase activity. Elimination of RNase H activity enhances the intrinsic thermal stability of the protein rather than its affinity to template-primer Moloney murine leukemia virus
D524A site-directed mutagenesis, RNase H-inactive mutant, that shows increased intrinsic thermal stability compared to the wild-type enzyme. The mutant loses RNase H activity through abolishing of Mg2+ binding to the RNase H domain Moloney murine leukemia virus
D583A site-directed mutagenesis, RNase H-inactive mutant, that shows increased intrinsic thermal stability compared to the wild-type enzyme. The mutant loses RNase H activity through abolishing of Mg2+ binding to the RNase H domain Moloney murine leukemia virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Moloney murine leukemia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Moloney murine leukemia virus Moloney murine leukemia virus reverse transcriptase, MMLV RT, shows DNA polymerization activity and RNase H activity. Stabilization of the reverse transcriptase activity by eliminating the RNase H activity, overview ?
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?

Organism

Organism UniProt Comment Textmining
Moloney murine leukemia virus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Moloney murine leukemia virus reverse transcriptase, MMLV RT, shows DNA polymerization activity and RNase H activity. Stabilization of the reverse transcriptase activity by eliminating the RNase H activity, overview Moloney murine leukemia virus ?
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?

Synonyms

Synonyms Comment Organism
RNase H
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Moloney murine leukemia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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assay at Moloney murine leukemia virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43.6
-
wild-type, 50% residual activity after a 10 min incubation Moloney murine leukemia virus
46.2
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wild-type, 50% residual activity after a 10 min incubation, presence of primer-template Moloney murine leukemia virus
47.3
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mutant D524A, 50% residual activity after a 10 min incubation Moloney murine leukemia virus
49.7
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mutant D524A, 50% residual activity after a 10 min incubation, presence of primer-template Moloney murine leukemia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Moloney murine leukemia virus

General Information

General Information Comment Organism
additional information catalytically important residues of the RNase H activity are Asp524 and Asp583 Moloney murine leukemia virus