Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Moloney murine leukemia virus |
Protein Variants | Comment | Organism |
---|---|---|
D524A | mutant lacks RNase H activity, but retains reverse transcriptase activity. Elimination of RNase H activity enhances the intrinsic thermal stability of the protein rather than its affinity to template-primer | Moloney murine leukemia virus |
D524A | site-directed mutagenesis, RNase H-inactive mutant, that shows increased intrinsic thermal stability compared to the wild-type enzyme. The mutant loses RNase H activity through abolishing of Mg2+ binding to the RNase H domain | Moloney murine leukemia virus |
D583A | site-directed mutagenesis, RNase H-inactive mutant, that shows increased intrinsic thermal stability compared to the wild-type enzyme. The mutant loses RNase H activity through abolishing of Mg2+ binding to the RNase H domain | Moloney murine leukemia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Moloney murine leukemia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Moloney murine leukemia virus | Moloney murine leukemia virus reverse transcriptase, MMLV RT, shows DNA polymerization activity and RNase H activity. Stabilization of the reverse transcriptase activity by eliminating the RNase H activity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Moloney murine leukemia virus reverse transcriptase, MMLV RT, shows DNA polymerization activity and RNase H activity. Stabilization of the reverse transcriptase activity by eliminating the RNase H activity, overview | Moloney murine leukemia virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Moloney murine leukemia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Moloney murine leukemia virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43.6 | - |
wild-type, 50% residual activity after a 10 min incubation | Moloney murine leukemia virus |
46.2 | - |
wild-type, 50% residual activity after a 10 min incubation, presence of primer-template | Moloney murine leukemia virus |
47.3 | - |
mutant D524A, 50% residual activity after a 10 min incubation | Moloney murine leukemia virus |
49.7 | - |
mutant D524A, 50% residual activity after a 10 min incubation, presence of primer-template | Moloney murine leukemia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Moloney murine leukemia virus |
General Information | Comment | Organism |
---|---|---|
additional information | catalytically important residues of the RNase H activity are Asp524 and Asp583 | Moloney murine leukemia virus |