Literature summary for 3.1.26.13 extracted from

Mizrahi, V.
Analysis of the ribonuclease H activity of HIV-1 reverse transcriptase using RNA-DNA hybrid substrates derived from the gag region of HIV-1 (1989), Biochemistry, 28, 9088-9094.

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Organism

Organism	UniProt	Comment	Textmining
Human immunodeficiency virus 1	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrates consist of SP6 runoff transcripts from a portion of the gag region of the HIV-1 genome hybridized to complementary single-stranded DNA from either an M 13 subclone or a phagemid transcription vector subclone. The corresponding hybrids are fully base-paired	Human immunodeficiency virus 1	?	products formed from the fully complementary hybrid consist of a nonuniform distribution of oligonucleotides ranging in size from 4 to 15 nt	?
additional information	substrates consist of SP6 runoff transcripts from a portion of the gag region of the HIV-1 genome hybridized to complementary single-stranded DNA from either an M 13 subclone or a phagemid transcription vector subclone. The corresponding hybrids carry a 5'-mismatch of seven nucleotides	Human immunodeficiency virus 1	?	products are a few prominent intermediates of 24-42 nt in size with relatively little accumulation of larger products	?

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Release 2023.1 (January 2023)