Crystallization (Comment) | Organism |
---|---|
apoprotein, X-ray diffraction structure determination and analysis at 3.3 resolution, modelling using molecular replacement | Escherichia coli |
RNase E catalytic domain in the apo-state, molecular replacement | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the magnesium ion is co-recruited with RNA during ligand binding | Escherichia coli | |
Zn2+ | conformation and activity of apo- and holoenzyme, modelling, overview | Escherichia coli | |
Zn2+ | the enzyme contains Zn2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | RNase E is an essential bacterial endoribonuclease involved in the turnover of messenger RNA and the maturation of structured RNA precursors in Escherichia coli, RNA degradation mechanism, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21513 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNase E is an essential bacterial endoribonuclease involved in the turnover of messenger RNA and the maturation of structured RNA precursors in Escherichia coli, RNA degradation mechanism, overview | Escherichia coli | ? | - |
? | |
additional information | RNA degradation mechanism, overview | Escherichia coli | ? | - |
? | |
additional information | RNase E preferres substrates possessing a 5'-monophosphate | Escherichia coli | ? | - |
? | |
additional information | upon catalytic activation, RNase E undergoes a marked conformational change characterized by the coupled movement of two RNA-binding domains to organize the active site | Escherichia coli | ? | - |
? | |
mRNA + H2O | - |
Escherichia coli | ? | - |
? | |
tRNAPhe precursor + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | dimer of dimers, RNase E apoprotein, X-ray crystallography | Escherichia coli |
tetramer | a pair of dimers, the quaternary organization of RNase E is flexible, modelling, apoprotein formation leads to a conformational change in which the 5' sensor and S1 subdomains move as a single unit through an angle between the apoprotein and holoprotein state, overview | Escherichia coli |
tetramer | RNase E holoenzyme, X-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |