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Literature summary for 3.1.26.12 extracted from

  • Schein, A.; Sheffy-Levin, S.; Glaser, F.; Schuster, G.
    The RNase E/G-type endoribonuclease of higher plants is located in the chloroplast and cleaves RNA similarly to the E. coli enzyme (2008), RNA, 14, 1057-1068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.12

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
EDTA disrupts the oligomer Arabidopsis thaliana
structured RNA inhibits the plant enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast soluble fraction Arabidopsis thaliana 9507
-
additional information no enzyme in the mitochondria Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, lack of Mg2+ leads to unspecific cleavage of pSu3 RNA to small oligoribonucleotides Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 x * 115000, about, sequence calculation, x * 150000-160000, SDS-PAGE Arabidopsis thaliana
680000
-
 gel filtration, the 680 kDa complex is resistant to a high salt concentration of up to 2 M KCl , but is disrupted by 10 mM EDTA Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana RNA degradation in the chloroplast occurs via the polyadenylation-assisted degradation pathway, plant RNase E participates in the initial endonucleolytic cleavage of the polyadenylation-stimulated RNA degradation process in the chloroplast, perhaps in collaboration with the two other chloroplast endonucleases, RNase J and CSP41, overview ?
-
 ?
pSu3 + H2O Arabidopsis thaliana endonucleolytic cleavage, the precursor of the Escherichia coli tRNATyrSu3, cleavage upstream of the RNase P cleavage site in vitro and in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13mer nucleotide sequence of RNAI + H2O endonucleolytic cleavage, a synthetic 13-nt oligoribonucleotide, representing the cleavage site of RNAI, from the 5' end, with the canonical RNase E cleavage site located between U5 and A6 Arabidopsis thaliana ?
-
 ?
additional information RNA degradation in the chloroplast occurs via the polyadenylation-assisted degradation pathway, plant RNase E participates in the initial endonucleolytic cleavage of the polyadenylation-stimulated RNA degradation process in the chloroplast, perhaps in collaboration with the two other chloroplast endonucleases, RNase J and CSP41, overview Arabidopsis thaliana ?
-
 ?
pSu3 + H2O endonucleolytic cleavage, the precursor of the Escherichia coli tRNATyrSu3, cleavage upstream of the RNase P cleavage site in vitro and in vivo Arabidopsis thaliana ?
-
 ?
pSu3 + H2O endonucleolytic cleavage, the precursor of the Escherichia coli tRNATyrSu3, cleavage upstream of the RNase P cleavage site in vitro and in vivo, cleavage site mapping, overview, very low activity with the substrate mutants K546A and K552A, lack of Mg2+ leads to unspecific cleavage of pSu3 RNA to small oligoribonucleotides Arabidopsis thaliana ?
-
 ?
RNA + H2O endonucleolytic cleavage, the Arabidopsis enzyme uses single-stranded oligoribonucleotide and chloroplast RNA as substrates, and depends on the number of phosphates at the 5' end, is inhibited by structured RNA, and preferentially cleaves A/U-rich sequences, catalytic domain structure, overview Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
More catalytic domain structure, homology-based modelling, overview Arabidopsis thaliana
oligomer x * 115000, about, sequence calculation, x * 150000-160000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
RNase E
-
 Arabidopsis thaliana
RNase E/G-type endoribonuclease
-
 Arabidopsis thaliana