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Literature summary for 3.1.26.12 extracted from

  • Kaberdin, V.R.
    Probing the substrate specificity of Escherichia coli RNase E using a novel oligonucleotide-based assay (2003), Nucleic Acids Res., 31, 4710-4716.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.12

Application

Application Comment Organism
analysis the enzyme can be a model for investigation of the action of site specific nucleases Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene rne, expression of the isolated, His-tagged catalytic domain Rne498, comprising residues 1-498, in strain BL21(DE3) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isolated, His-tagged catalytic domain Rne498 from strain BL21(DE3) by metal affinity chromatography and dialysis to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 validation of an oligonucleotide-based assay method, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-labeled RNA oligonucleotides + H2O synthetic RNA substrate variants based on known enzyme RNA substrate sequences, recombinant Rne498 catalytic domain, cleavage site specificity, overview Escherichia coli ?
-
 ?
5'AUCAAAGAAA + H2O 5'-labeled synthetic RNA substrate, modified 9S RNA sequence, recombinant Rne498 catalytic domain, no activity with the wild-type 9S RNA sequence 5'AUCAAAUAAA and with modified sequence 5'AUCAGAUAAA Escherichia coli 5'AUCAAAGA + AA
-
 ?
5'AUCAAGUAAA + H2O low activity, 5'-labeled synthetic RNA substrate, modified 9S RNA sequence, recombinant Rne498 catalytic domain, no activity with the wild-type 9S RNA sequence 5'AUCAAAUAAA and with modified sequence 5'AUCAGAUAAA Escherichia coli 5'AUCAAGU + AAA
-
 ?
5'AUCGAAUAAA + H2O 5'-labeled synthetic RNA substrate, modified 9S RNA sequence, recombinant Rne498 catalytic domain, no activity with the wild-type 9S RNA sequence 5'AUCAAAUAAA and with modified sequence 5'AUCAGAUAAA Escherichia coli 5'AUCGA + AUAAA
-
 ?
additional information identification of specific sequence determinants that either facilitate or impede the recognition and cleavage of RNA by the enzyme, RNA-enzyme interactions, overview Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
More RNase E is a multi-domain enzyme Escherichia coli

Synonyms

Synonyms Comment Organism
RNase E
-
 Escherichia coli