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Literature summary for 3.1.26.12 extracted from

  • Mackie, G.A.
    Ribonuclease E is a 5'-end-dependent endonuclease (1998), Nature, 395, 720-723.
    View publication on PubMed
Search for more literature for 3.1.26.12

Inhibitors

Inhibitors Comment Organism Structure
additional information anti-sense deoxynucleotide constructs complementary to the 5' end sequences of RNA substrates reduce the enzyme activity Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme is localized in a multi-protein complex, the RNA degradosome Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
9S rRNA precursor + H2O Escherichia coli
-
 5S rRNA + ?
-
 ?
9S rRNA precursor + H2O Escherichia coli CF 881
-
 5S rRNA + ?
-
 ?
rpsT mRNA + H2O Escherichia coli the substrate encodes the ribosomal protein S20 ?
-
 ?
rpsT mRNA + H2O Escherichia coli CF 881 the substrate encodes the ribosomal protein S20 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene rne
-
Escherichia coli CF 881
-
 gene rne
-

Purification (Commentary)

Purification (Comment) Organism
native degradosomes from strain CF881, which lacks RNase I, further electrophoretically purification of Rne protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9S rRNA precursor + H2O
-
 Escherichia coli 5S rRNA + ?
-
 ?
9S rRNA precursor + H2O very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview Escherichia coli 5S rRNA + ?
-
 ?
9S rRNA precursor + H2O
-
 Escherichia coli CF 881 5S rRNA + ?
-
 ?
9S rRNA precursor + H2O very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview Escherichia coli CF 881 5S rRNA + ?
-
 ?
additional information ribonuclease E is a 5'-end-dependent, single-strand-specific endonuclease that initiates the selective decay of mRNA having regulatory function, regulation of mRNA levels in response to environment, the enzyme proceeds in a 5' to 3' direction, the enzyme shows high activity with 5' monophosphorylated RNA, but low activity with 5' triphosphorylated RNA, poor activity with circular RNA substrates, overview Escherichia coli ?
-
 ?
additional information ribonuclease E is a 5'-end-dependent, single-strand-specific endonuclease that initiates the selective decay of mRNA having regulatory function, regulation of mRNA levels in response to environment, the enzyme proceeds in a 5' to 3' direction, the enzyme shows high activity with 5' monophosphorylated RNA, but low activity with 5' triphosphorylated RNA, poor activity with circular RNA substrates, overview Escherichia coli CF 881 ?
-
 ?
rpsT mRNA + H2O the substrate encodes the ribosomal protein S20 Escherichia coli ?
-
 ?
rpsT mRNA + H2O the substrate encodes the ribosomal protein S20, very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview Escherichia coli ?
-
 ?
rpsT mRNA + H2O the substrate encodes the ribosomal protein S20 Escherichia coli CF 881 ?
-
 ?
rpsT mRNA + H2O the substrate encodes the ribosomal protein S20, very low activity with a covalently closed circular variant of the substrate compared to the linear one, overview Escherichia coli CF 881 ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme is localized in a multi-protein complex, the RNA degradosome Escherichia coli

Synonyms

Synonyms Comment Organism
ribonuclease E
-
 Escherichia coli
RNase E
-
 Escherichia coli
Rne protein
-
 Escherichia coli