Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F57A | site-directed mutagenesis, 91% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
F67A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K106A | site-directed mutagenesis, 60% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K112A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K37A | site-directed mutagenesis, 94% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K38A | site-directed mutagenesis, 49% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K43A | site-directed mutagenesis, 33% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
K71A | site-directed mutagenesis, 56% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
R109A | site-directed mutagenesis, 78% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
R48A | site-directed mutagenesis, 49% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
R64A | site-directed mutagenesis, 77% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
R87A | site-directed mutagenesis, 16% increased feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
R95A | site-directed mutagenesis, 19% increased feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
Y42A | site-directed mutagenesis, 48% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
Y60A | site-directed mutagenesis, 99% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
Y77A | site-directed mutagenesis, 19% reduced feedback regulation activity compared to the wild-type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme, especially its catalytic N-terminal domain, is essential for RNA processing and degradation, and for cell growth and feedback regulation of RNase E synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
feedback regulation activity of wild-type and mutant enzymes, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | reaction mechanism | Escherichia coli | ? | - |
? | |
additional information | the enzyme, especially its catalytic N-terminal domain, is essential for RNA processing and degradation, and for cell growth and feedback regulation of RNase E synthesis | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional enzyme structure with an essential N-terminal RNase E domain of the S1 family RNA-binding domain fold, the residues involved in cell growth and feedback regulation of RNase E synthesis form two cluster, e.g. Phe57, Phe67, and Tyr112, or Lys37 and Tyr60, overview, structural modeling of the S1 domain | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the S1 family of RNA-binding enzymes | Escherichia coli |
RNase E | - |
Escherichia coli |