Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.12 extracted from

  • Cohen, S.N.; McDowall, K.J.
    RNase E: still a wonderfully mysterious enzyme (1997), Mol. Microbiol., 23, 1099-1106.
    View publication on PubMed
Search for more literature for 3.1.26.12

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of plasmid-encoded rne gene increases cell doubling time and can lead to plasmid loss or aquisition of mutations that reduce RNase E activity Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information rne is an essential gene, its overexpression interferes with cell growth and viability Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-
additional information the enzyme is also part of a high-molecular-weight degradosome, which also contains polynucleotide phosphorylase, but lacks RNase P and RNase III Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 x * 180000, SDS-PAGE, x * 118000, sequence calculation Escherichia coli
180000
-
 x * 180000, SDS-PAGE, x * 118000, sequence calculation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli RNase E is involved in and interacts with functionally and physically polynucleotide phosphorylase, and also with other enzymes implicated in the processing and degradation of RNA, polynuclease phosphorylase, PNPase, degrades the reaction products generated by RNase E ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene rne, also known as ams or hmp
-
Rhodobacter capsulatus
-
-
-
Streptomyces lividans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9S RNA + H2O cleavage site specificity, overview Escherichia coli ?
-
 ?
additional information RNase E is involved in and interacts with functionally and physically polynucleotide phosphorylase, and also with other enzymes implicated in the processing and degradation of RNA, polynuclease phosphorylase, PNPase, degrades the reaction products generated by RNase E Escherichia coli ?
-
 ?
additional information cleavage site specificity, overview Rhodobacter capsulatus ?
-
 ?
additional information cleavage site specificity, overview Streptomyces lividans ?
-
 ?
additional information the N-terminal catalytic domain is sufficient for catalytic activity, the enzyme shows high RNA binding ability, and cleavage of mRNA and rRNA, RNase E interacts with polynucleotide phosphorylase and other enzymes implicated in the processing and degradation of RNA, cleavage site specificity, overview, RNase E requires the stem loop structure in RNA substrates, altering of the substrate secondary structure alters substrate specificity, overview, the enzymes' arginine-rich RNA-binding site is not essential for activity but allows the degradosome to move progressively along the transcript during degradation Escherichia coli ?
-
 ?
RNA I + H2O full-length RNA I by plasmids pBR322 or pACY184, cleavage site specificity, overview Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE, x * 118000, sequence calculation Escherichia coli
More the Rne protein is composed of an N-terminal catalytic domain, two proline-rich segments, an arginine-rich RNA-binding site ARRBS segment, and a proline-rich and acidic domain, domain organization, overview Escherichia coli

Synonyms

Synonyms Comment Organism
ribonuclease E
-
 Escherichia coli
ribonuclease E
-
 Rhodobacter capsulatus
ribonuclease E
-
 Streptomyces lividans
RNase E
-
 Escherichia coli
RNase E
-
 Rhodobacter capsulatus
RNase E
-
 Streptomyces lividans
Rne protein
-
 Escherichia coli