Cloned (Comment) | Organism |
---|---|
expression of His-tagged N-terminal catalytic domain in strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, purified recombinant His-tagged N-terminal catalytic domain | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal at 20 mM in a buffer containing 100 mM NaCl and 0.1% v/v Triton X-100 | Escherichia coli | |
NaCl | activates the catalytic enzyme domain, optimally at 150 mM | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme is required for rapid decay and correct hydrolytic processing of RNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged N-terminal catalytic domain from strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5' monophosphorylated RNA oligonucleotides + H2O | several synthetic substrates, overview | Escherichia coli | ? | - |
? | |
additional information | the enzyme is required for rapid decay and correct hydrolytic processing of RNA | Escherichia coli | ? | - |
? | |
additional information | RNA processing reaction mechanism and involved functional groups, activity depends on protonated and unprotonated groups, the recognition of a guanosine sequence determinant upstream of the scissile bond via interaction with the exocyclic 2-amino group, the 7N of the nucleobase, and the imino proton or 6-keto group, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease E | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | inactive at pH 6.5 and pH 10.5 | Escherichia coli |