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Literature summary for 3.1.26.12 extracted from

  • Redko, Y.; Tock, M.R.; Adams, C.J.; Kaberdin, V.R.; Grasby, J.A.; McDowall, K.J.
    Determination of the catalytic parameters of the N-terminal half of Escherichia coli ribonuclease E and the identification of critical functional groups in RNA substrates (2003), J. Biol. Chem., 278, 44001-44008.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged N-terminal catalytic domain in strain BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, purified recombinant His-tagged N-terminal catalytic domain Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal at 20 mM in a buffer containing 100 mM NaCl and 0.1% v/v Triton X-100 Escherichia coli
NaCl activates the catalytic enzyme domain, optimally at 150 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the enzyme is required for rapid decay and correct hydrolytic processing of RNA ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged N-terminal catalytic domain from strain BL21(DE3) by nickel affinity chromatography and gel filtration to over 95% purity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5' monophosphorylated RNA oligonucleotides + H2O several synthetic substrates, overview Escherichia coli ?
-
?
additional information the enzyme is required for rapid decay and correct hydrolytic processing of RNA Escherichia coli ?
-
?
additional information RNA processing reaction mechanism and involved functional groups, activity depends on protonated and unprotonated groups, the recognition of a guanosine sequence determinant upstream of the scissile bond via interaction with the exocyclic 2-amino group, the 7N of the nucleobase, and the imino proton or 6-keto group, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ribonuclease E
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 inactive at pH 6.5 and pH 10.5 Escherichia coli