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Literature summary for 3.1.26.12 extracted from
- RNase E cleaves at multiple sites in bubble regions of RNA I stem loops yielding products that dissociate differentially from the enzyme (1996), J. Biol. Chem., 271, 13103-13109.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli K-12 |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | - |
strains N3433 and N3431 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme can cleave internucleotide bonds in the bubble regions of duplex RNA segments and in single-stranded regions, mechanism | Escherichia coli K-12 | ? | - |
? | |
| RNA I + H2O | full-length RNA I and GGGRNA I encoded by plasmids pBR322, pCML103, or pCML108, RNA binding domain structure, secondary structure of the substrate RNA, complex formation and mechanism, multiple cleavage sites, overview | Escherichia coli K-12 | ? | several fragments, product mapping, overview | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase E | - |
Escherichia coli K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli K-12 |
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