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Literature summary for 3.1.26.12 extracted from

  • Kim, K.S.; Sim, S.; Ko, J.H.; Cho, B.; Lee, Y.
    Kinetic analysis of precursor M1 RNA molecules for exploring substrate specificity of the N-terminal catalytic half of RNase E (2004), J. Biochem., 136, 693-699.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene rne, expression of His-tagged N-terminal half of RNase E in strain BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
UUUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00032
-
UUUUU-DN34-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00037
-
GUUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00046
-
CAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00073
-
AAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00081
-
UAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.00124
-
GAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene gld
-
Escherichia coli JM109
-
gene gld
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged N-terminal half of RNase E from strain BL21(DE3) Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AAUUU-containing RNA oligonucleotide + H2O G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?
AAUUU-containing RNA oligonucleotide + H2O G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli JM109 ?
-
?
CAUUU-containing RNA oligonucleotide + H2O G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?
CAUUU-containing RNA oligonucleotide + H2O G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli JM109 ?
-
?
GAUUU-containing RNA oligonucleotide + H2O wild-type substrate, best substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?
GAUUU-containing RNA oligonucleotide + H2O wild-type substrate, best substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli JM109 ?
-
?
GUUUU-containing RNA oligonucleotide + H2O A379 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?
GUUUU-containing RNA oligonucleotide + H2O A379 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli JM109 ?
-
?
additional information analysis of cleavage site specficity Escherichia coli ?
-
?
additional information analysis of cleavage site specficity Escherichia coli JM109 ?
-
?
UAUUU-containing RNA oligonucleotide + H2O G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?
UUUUU-containing RNA oligonucleotide + H2O G378/A379 mutant substrate, p23 RNA variant derived from linearized DraI DN1 or DN34 plasmids, in vitro substrate synthesis by SP6 RNA poylmerase Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
RNase E
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
UUUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.023
-
UUUUU-DN34-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.121
-
GUUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.15
-
CAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.192
-
AAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.209
-
UAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli
0.765
-
GAUUU-containing RNA oligonucleotide recombinant enzyme N-terminal half, pH 7.5, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli