Cloned (Comment) | Organism |
---|---|
gene rne, expression of His-tagged N-terminal half of RNase E in strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
UUUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00032 | - |
UUUUU-DN34-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00037 | - |
GUUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00046 | - |
CAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00073 | - |
AAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00081 | - |
UAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.00124 | - |
GAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene gld | - |
Escherichia coli JM109 | - |
gene gld | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged N-terminal half of RNase E from strain BL21(DE3) | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AAUUU-containing RNA oligonucleotide + H2O | G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? | |
AAUUU-containing RNA oligonucleotide + H2O | G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli JM109 | ? | - |
? | |
CAUUU-containing RNA oligonucleotide + H2O | G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? | |
CAUUU-containing RNA oligonucleotide + H2O | G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli JM109 | ? | - |
? | |
GAUUU-containing RNA oligonucleotide + H2O | wild-type substrate, best substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? | |
GAUUU-containing RNA oligonucleotide + H2O | wild-type substrate, best substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli JM109 | ? | - |
? | |
GUUUU-containing RNA oligonucleotide + H2O | A379 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? | |
GUUUU-containing RNA oligonucleotide + H2O | A379 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli JM109 | ? | - |
? | |
additional information | analysis of cleavage site specficity | Escherichia coli | ? | - |
? | |
additional information | analysis of cleavage site specficity | Escherichia coli JM109 | ? | - |
? | |
UAUUU-containing RNA oligonucleotide + H2O | G378 mutant substrate, p23 RNA variant derived from linearized DraI plasmid, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? | |
UUUUU-containing RNA oligonucleotide + H2O | G378/A379 mutant substrate, p23 RNA variant derived from linearized DraI DN1 or DN34 plasmids, in vitro substrate synthesis by SP6 RNA poylmerase | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
UUUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.023 | - |
UUUUU-DN34-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.121 | - |
GUUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.15 | - |
CAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.192 | - |
AAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.209 | - |
UAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli | |
0.765 | - |
GAUUU-containing RNA oligonucleotide | recombinant enzyme N-terminal half, pH 7.5, 30°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |