Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | a monophosphate at the 5' end of the RNA substrate stimulates the enzyme | Escherichia coli K-12 |
Cloned (Comment) | Organism |
---|---|
gene rne, overexpression in Escherichia coli, functional complementation of the RNase E-deficient strain CJ1832 of Escherichia coli | Synechocystis sp. |
overexpression of active His- and Myc-tagged truncated enzyme in strain BL21(DE3) | Escherichia coli K-12 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the truncated mutant enzyme N-RNase E consisting of the N-terminal catalytic site, residues 1-498, an enzyme-deficient strain CJ1832 can be complemented by expression of SynRne of Synechocystis sp., but not by Escherichia coli CafA, i.e. RNase G | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Synechocystis sp. | |
Mg2+ | - |
Escherichia coli K-12 | |
NH4+ | enzyme assay in presence of 0.1 M NH4Cl | Synechocystis sp. | |
NH4+ | enzyme assay in presence of 0.1 M NH4Cl | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | Escherichia coli K-12 | the enzyme plays a key role in processing and degradation of RNA in Escherichia coli | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
strains CJ1827 and CJ1828 derived from strain MC1061, gene rne or ams | - |
Synechocystis sp. | - |
strain PCC 6803, gene rne | - |
Purification (Comment) | Organism |
---|---|
recombinant active His- and Myc-tagged truncated enzyme from strain BL21(DE3) by affinity chromatography | Escherichia coli K-12 |
recombinant enzyme from Escherichia coli | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity of different strains, comparison of wild-type RNase E and mutant N-RNase E to SynRne of Synechocystis sp., overview | Escherichia coli K-12 |
additional information | - |
relative activity of SynRne expressed in Escherichia coli compared to the Escherichia coli enzyme, overview | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-capped RNA I.26 + H2O | low activity, cleavage of the 5' substrate end | Escherichia coli K-12 | ? | - |
? | |
9S rRNA + H2O | - |
Synechocystis sp. | ? | - |
? | |
pppRNA I.26 + H2O | low activity, cleavage of the 5' substrate end | Escherichia coli K-12 | ? | - |
? | |
pRNA I.26 + H2O | a monophosphate at the 5' end of the RNA I substrate stimulates the enzyme 25-30fold, cleavage of the 5' substrate end | Escherichia coli K-12 | ? | - |
? | |
RNA + H2O | the enzyme plays a key role in processing and degradation of RNA in Escherichia coli | Escherichia coli K-12 | ? | - |
? | |
RNA I + H2O | - |
Synechocystis sp. | ? | - |
? | |
RNA I + H2O | cleavage of the 5' substrate end | Escherichia coli K-12 | ? | - |
? | |
RNA I.26 + H2O | - |
Synechocystis sp. | ? | - |
? | |
RNA I.26 + H2O | cleavage of the 5' substrate end | Escherichia coli K-12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the catalytic site is located at the N-terminus, residues 1-406 | Synechocystis sp. |
More | the catalytic site is located at the N-terminus, residues 1-413 | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Synechocystis sp. |
RNase E | - |
Escherichia coli K-12 |
SynRne | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechocystis sp. |
30 | - |
assay at | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Synechocystis sp. |
7.5 | - |
assay at | Escherichia coli K-12 |