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Literature summary for 3.1.26.11 extracted from

  • Minagawa, A.; Takaku, H.; Shibata, H.; Ishii, R.; Takagi, M.; Yokoyama, S.; Nashimoto, M.
    Substrate recognition ability differs among various prokaryotic tRNase Zs (2006), Biochem. Biophys. Res. Commun., 345, 385-393 .
    View publication on PubMed
Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus
expression of the histidine-tagged enzyme in Escherichia coli Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Bacillus subtilis pre-tRNACys is cleaved in the presence of Mn2+ but not in the presence of Mg2+ Bacillus subtilis
Mn2+ Bacillus subtilis pre-tRNACys is cleaved in the presence of Mn2+ but not in the presence of Mg2+ Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P54548
-
-
Bacillus subtilis 168 P54548
-
-
Pyrobaculum aerophilum Q8ZTJ7
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZTJ7
-
-
Thermoplasma acidophilum Q9HJ19
-
-
Thermotoga maritima Q9WY50
-
-
Thermotoga maritima DSM 3109 Q9WY50
-
-
Thermus thermophilus Q53W74
-
-
Thermus thermophilus DSM 579 Q53W74
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bacillus subtilis pre-tRNATrp + H2O cleaved well after C75 relatively Thermus thermophilus ?
-
 ?
Bacillus subtilis pre-tRNATrp + H2O cleaved well after C75 relatively Thermus thermophilus DSM 579 ?
-
 ?
Bacillus subtilis tRNACys + H2O efficient cleavage Bacillus subtilis ?
-
 ?
Bacillus subtilis tRNACys + H2O efficient cleavage Bacillus subtilis 168 ?
-
 ?
Escherichia coli pre-tRNAPhe + H2O cleavage is barely detected Thermus thermophilus ?
-
 ?
Escherichia coli pre-tRNAPhe + H2O cleavage is barely detected Thermus thermophilus DSM 579 ?
-
 ?
human pre-tRNAArg + H2O efficient cleavage Bacillus subtilis ?
-
 ?
human pre-tRNAArg + H2O efficient cleavage Thermoplasma acidophilum ?
-
 ?
human pre-tRNAArg + H2O efficient cleavage Bacillus subtilis 168 ?
-
 ?
Thermoplasma acidophilum pre-tRNAPhe + H2O weak cleavage Thermoplasma acidophilum ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O cleavage is barely detected Thermus thermophilus ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75 Thermotoga maritima ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O weak cleavage Pyrobaculum aerophilum ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O weak cleavage Pyrobaculum aerophilum DSM 7523 ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O cleaved very inefficiently. The cleavage site of pre-tRNAArg(CCA) is identified after C75 Thermotoga maritima DSM 3109 ?
-
 ?
Thermotoga maritima pre-tRNAArg(CCA) + H2O cleavage is barely detected Thermus thermophilus DSM 579 ?
-
 ?
Thermotoga maritima pre-tRNAArg(GUG) + H2O efficient cleavage Bacillus subtilis ?
-
 ?
Thermotoga maritima pre-tRNAArg(GUG) + H2O cleaved very inefficiently Thermotoga maritima ?
-
 ?
Thermotoga maritima pre-tRNAArg(GUG) + H2O weak cleavage Thermoplasma acidophilum ?
-
 ?
Thermotoga maritima pre-tRNAArg(GUG) + H2O efficient cleavage Bacillus subtilis 168 ?
-
 ?
Thermotoga maritima pre-tRNAArg(GUG) + H2O cleaved very inefficiently Thermotoga maritima DSM 3109 ?
-
 ?
Thermotoga maritima pre-tRNAMet(CCA) + H2O cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively Thermotoga maritima ?
-
 ?
Thermotoga maritima pre-tRNAMet(CCA) + H2O cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) are cleaved after C75 and after the discriminator, respectively Thermotoga maritima DSM 3109 ?
-
 ?
Thermotoga maritima pre-tRNAMet(UAG) + H2O cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively Thermotoga maritima ?
-
 ?
Thermotoga maritima pre-tRNAMet(UAG) + H2O cleaved albeit very inefficiently. Pre-tRNAMet(CCA) and pre-tRNAMet(UAG) were cleaved after C75 and after the discriminator, respectively Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration analysis shows that, only 20% of tRNase Z2 molecules form dimers, while tRNase Z molecules exist as dimers. This inefficient tRNase Z2 dimer formation may be attributed to the lack of alpha1, alpha2, and alpha3 that are important for dimerization of tRNase Z Thermotoga maritima

Synonyms

Synonyms Comment Organism
ribonuclease Z
-
 Thermoplasma acidophilum
ribonuclease Z
-
 Pyrobaculum aerophilum
TM0207
-
 Thermotoga maritima
tRNase Z2
-
 Thermotoga maritima
YP_145327
-
 Thermus thermophilus