Cloned (Comment) | Organism |
---|---|
PCR products digested with XhoI/XmaI, and cloned into the XhoI/XmaI sites of pREP4X and pREP82X, creating pREP4X-f-TRZ1 and pREP82X-f-TRZ1, respectively. Overexpressed in sla1+ (yYH1) or isogenetic sla1DELTA (yAS110) cells. FLAG-tagged wild-type or untagged Trz1 overexpressed in the heterozygous diploid Schizosaccharomyces pombe strain trz1+/trz1DELTA | Saccharomyces cerevisiae |
PCR products for trz1+ digested with SalI and XmaI, and cloned between the SalI/XmaI sites of pREP4X (the full strength thiamine-repressible nmt1+ promoter) and the XhoI/XmaI sites pREP82X (the lowstrength thiamine-repressible nmt1+ promoter), yielding pREP4X-f-trz1 and pREP82X-f-trz1, respectively. Overexpression of wild-type trz1+ and mutants in yYH1 cells or in an isogenetic sla1DELTA strain (yAS110) | Schizosaccharomyces pombe |
PCR products for trz2+ digested with XhoI and XmaI, and cloned into the XhoI and XmaI sites of pREP82X and pREP4X, producing pREP82X-f-trz2 and pREP4X-f-trz2, respectively. PCR products for the 5' and 3' flanks of trz2+ cloned into the SacI/PstI sites and SalI/KpnI sites of pAF1, creating pAF1-trz2int | Schizosaccharomyces pombe |
PCR products of ELAC1 digested with XhoI and BamHI, and cloned between the XhoI/BamHI sites of pREP4X, producing pREP4X-f-ELAC1. PCR products of ELAC2 digested with XhoI and XmaI, and cloned between the XhoI/XmaI sites of pREP4X, generating pREP4X-f-ELAC2, pREP4X-f-ELAC2-S217L, pREP4X-f-ELAC2-A541T, pREP4X-f-ELAC2-R781H and pREP4X-f-ELAC2-1641insG, respectively. Overexpressed in sla1+ (yYH1) or isogenetic sla1DELTA (yAS110) cells. FLAG-tagged wild-type or mutants overproduced in the heterozygous diploid Schizosaccharomyces pombe strain trz1+/trz1DELTA | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
1641insG | frameshift mutation of ELAC2, lacks the C-terminal half, cannot increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
A541T | overexpression of this missense mutant of ELAC2 can increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
D476A | overexpression of Trz2 in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1 | Schizosaccharomyces pombe |
D578A | overexpression in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1 | Schizosaccharomyces pombe |
H546A | ELAC2 mutation, cannot increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
H574A | overexpression in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1 | Schizosaccharomyces pombe |
I572K | inactivates the protein | Schizosaccharomyces pombe |
R781H | overexpression of this missense mutant of ELAC2 can increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
S217L | overexpression of this missense mutant of ELAC2 can increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
Y571L | mutation does not result in a temperature-sensitive phenotype | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | recombinant ELAC2 is localized to the nucleus of Schizosaccharomyces pombe | Homo sapiens | - |
- |
additional information | the N-terminus of Trz2p contains a mitochondrial targeting signal (aa 1-38) | Schizosaccharomyces pombe | - |
- |
additional information | Trz1p does not appear to contain a nuclear localization signal | Schizosaccharomyces pombe | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75900 | - |
calculated from sequence | Schizosaccharomyces pombe |
90600 | - |
calculated from sequence | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | P87168 | - |
- |
Schizosaccharomyces pombe | Q10155 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | overexpression of ELAC2 does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC) | Homo sapiens | ? | - |
? | |
additional information | overexpression of trz1+ does not appreciably increase the levels of mature tRNALys(CUU) and tRNAVal(AAC) | Schizosaccharomyces pombe | ? | - |
? | |
precursor tRNASer(UGA)-M + H2O | overexpression of ELAC2 increases the abundance of mature tRNASer(UGA)-M in strain yYH1 | Homo sapiens | mature tRNASer(UGA)-M | - |
? | |
precursor tRNASer(UGA)-M + H2O | overexpression of wild-type trz1+ in yYH1 cells can cause an increase in nonsense suppression by tRNASer(UGA)-M1, but overexpression of trz1+ cannot overcome the requirement for Sla1p for maturation of tRNASer(UGA)-M | Schizosaccharomyces pombe | mature tRNASer(UGA)-M | - |
? | |
precursor tRNASer(UGA)-M + H2O | overexpression of untagged TRZ1, but not FLAG-tagged TRZ1, increases the abundance of mature tRNASer(UGA)-M in strain yYH1 | Saccharomyces cerevisiae | mature tRNASer(UGA)-M + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Elac1 | - |
Homo sapiens |
ELAC2 | - |
Homo sapiens |
tRNase ZL | - |
Homo sapiens |
tRNase ZL | - |
Saccharomyces cerevisiae |
tRNase ZL | - |
Schizosaccharomyces pombe |
TRZ1 | - |
Saccharomyces cerevisiae |
TRZ1 | - |
Schizosaccharomyces pombe |
TRZ2 | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
malfunction | ELAC2 lacking a putative mitochondrial targeting signal (aa 1-30), but not ELAC2 lacking a putative nuclear localization signal (aa 28-35) can increase suppression of ade6-704 in strain yYH1 | Homo sapiens |
physiological function | humans contain one tRNaseZL encoded by the prostate-cancer susceptibility gene ELAC2. Wild-type overproduced from pREP4X, but not from pREP82X, can increase suppression of the UGA nonsense mutation ade6-704 in strain yYH1, through facilitating 3' end processing of the defective suppressor tRNA that mediates suppression. This is dependent on its endonucleolytic activity and its putative nuclear localization signal. Overexpression of ELAC1 cannot increase suppression. ELAC2 can rescue a temperature-sensitive allele of Schizosaccharomyces pombe trz1+, trz1-1, but not the trz1 null mutant | Homo sapiens |
physiological function | Saccharomyces cerevisiae contains one tRNase ZL (long form) encoded by the trz1 gene. TRZ1 can complement a temperature-sensitive allele of Schizosaccharomyces pombe trz1+, trz1-1, but not the Schizosaccharomyces pombe trz1 null mutant. Overexpression of TRZ1 can increase suppression of the UGA nonsense mutation ade6-704 through facilitating 3' end processing of the defective suppressor tRNA that mediates suppression | Saccharomyces cerevisiae |
physiological function | Schizosaccharomyces pombe contains two tRNase ZL (long form), encoded by trz1+ and trz2+. Both trz1+ and trz2+ are essential for growth. Ectopic expression of FLAG-tagged trz1+ and trz2+ can fully complement trz1+ and trz2+ disruption, respectively. Overexpression of trz2+ cannot increase suppression | Schizosaccharomyces pombe |
physiological function | Schizosaccharomyces pombe contains two tRNase ZL (long form), encoded by trz1+ and trz2+. Both trz1+ and trz2+ are essential for growth. Trz1+ is required for cell viability in the absence of Sla1p, which is required for endonuclease-mediated maturation of pre-tRNA 3' ends. Overexpression of trz1+ can increase suppression of the UGA nonsense mutation ade6-704 through facilitating 3' end processing of the defective suppressor tRNA that mediates suppression, thus can promote defective tRNA 3' processing in vivo. Both Saccharomyces cerevisiae TRZ1 and human ELAC2 can complement a temperature-sensitive allele of Schizosaccharomyces pombe trz1+, trz1-1, but not the trz1 null mutant. Ectopic expression of FLAG-tagged trz1+ and trz2+ can fully complement trz1+ and trz2+ disruption, respectively. Wild-type cannot increase suppression in the absence of sla1+ | Schizosaccharomyces pombe |