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Literature summary for 3.1.26.11 extracted from
- Residues in the conserved His domain of fruit fly tRNase Z that function in catalysis are not involved in substrate recognition or binding (2005), J. Mol. Biol., 350, 189-199.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in insect SF9 cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A493T | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| A501T | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| D502T | processing efficiency reduced 7420fold compared to wild-type tRNase Z | Drosophila melanogaster |
| G506A | processing efficiency reduced 11fold compared to wild-type tRNase Z | Drosophila melanogaster |
| H498A | processing efficiency reduced 1950fold compared to wild-type tRNase Z | Drosophila melanogaster |
| H500A | processing efficiency reduced 3379fold compared to wild-type tRNase Z | Drosophila melanogaster |
| H503A | processing efficiency reduced 817fold compared to wild-type tRNase Z | Drosophila melanogaster |
| H504A | processing efficiency reduced 7fold compared to wild-type tRNase Z | Drosophila melanogaster |
| I494A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| I505A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| I508A | processing efficiency reduced 6fold compared to wild-type tRNase Z | Drosophila melanogaster |
| L491A | processing efficiency reduced 7fold compared to wild-type tRNase Z | Drosophila melanogaster |
| L499A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| L507A | processing efficiency reduced 6fold compared to wild-type tRNase Z | Drosophila melanogaster |
| Q492A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| S497A | processing efficiency reduced 495fold compared to wild-type tRNase Z | Drosophila melanogaster |
| V496A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
| Y495A | processing efficiency similar to wild-type tRNase Z | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000155 | - |
pre-tRNAHis | 25 mM Tris-HCl pH 7.0, 1 mM MgCl2, 1 mM DTT, 50 mM KCl at 28°C | Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82920 | - |
calculated from sequence | Drosophila melanogaster |
| 90000 | - |
SDS-PAGE | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q8MKW7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tag fusion, Ni-affinity chromatography | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNAHis + H2O | - |
Drosophila melanogaster | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tRNaseZ | - |
Drosophila melanogaster |
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Release 2023.1 (January 2023)
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