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Literature summary for 3.1.26.11 extracted from

  • Ceballos-Chavez, M.; Vioque, A.
    Sequence-dependent cleavage site selection by RNase Z from the cyanobacterium Synechocystis sp. PCC 6803 (2005), J. Biol. Chem., 280, 33461-33469.
    View publication on PubMed
Search for more literature for 3.1.26.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)pLys Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00047
-
 pre-tRNAGlu-CCUN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.
0.00096
-
 pre-tRNAGlu-UUAN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.
0.00155
-
 pre-tRNAGlu-CUAN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography Synechocystis sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Synechocystis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNAGlu-CCAN17 + H2O
-
 Synechocystis sp. ?
-
 ?
pre-tRNAGlu-CCUN17 + H2O
-
 Synechocystis sp. ?
-
 ?
pre-tRNAGlu-CUAN17 + H2O
-
 Synechocystis sp. ?
-
 ?
pre-tRNAGlu-UCAN17 + H2O
-
 Synechocystis sp. ?
-
 ?
pre-tRNAGlu-UUAN17 + H2O
-
 Synechocystis sp. ?
-
 ?
pre-tRNAGlu-UUUN17 + H2O
-
 Synechocystis sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase Z
-
 Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 pre-tRNAGlu-CCUN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.
0.28
-
 pre-tRNAGlu-CUAN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.
2.25
-
 pre-tRNAGlu-UUAN17 40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol Synechocystis sp.