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Literature summary for 3.1.26.10 extracted from

  • Lalioti, V.S.; Ballesta, J.P.G.; Fragoulis, E.G.
    Purification and characterization of a novel poly(U), poly(C) ribonuclease from Saccharomyces cerevisiae (1997), Biochim. Biophys. Acta, 1342, 62-72.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimum enzyme activity at 50-100 mM Saccharomyces cerevisiae
Mg2+ optimum enzyme activity at 0.3-0.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mRNA + H2O Saccharomyces cerevisiae
-
?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
yeast, strain W303alpha
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
final specific activity of approximately 1500000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 40°C from poly(U) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no catalytic activity observed with tRNA and double stranded substrates, little or no reaction with poly(A), poly(I), poly(G), poly(dG)xpoly(dG-dC), and poly(dG-dC)xpoly(dG-dC) Saccharomyces cerevisiae ?
-
?
mRNA + H2O
-
Saccharomyces cerevisiae ?
-
?
mRNA + H2O mRNA obtained from in vitro transcription Saccharomyces cerevisiae ?
-
?
poly(C) x (dG)12-18 polynucleotide template primer + H2O
-
Saccharomyces cerevisiae ?
-
?
rRNA + H2O 26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA Saccharomyces cerevisiae ?
-
?
synthetic homopolynucleotide + H2O acts on poly(U) and poly(C) with a higher affinity for the latter Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5
-
Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae
-
-
7.5