BRENDA - Enzyme Database show
show all sequences of 3.1.26.10

Purification and characterization of a novel poly(U), poly(C) ribonuclease from Saccharomyces cerevisiae

Lalioti, V.S.; Ballesta, J.P.G.; Fragoulis, E.G.; Biochim. Biophys. Acta 1342, 62-72 (1997)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
optimum enzyme activity at 50-100 mM
Saccharomyces cerevisiae
Mg2+
optimum enzyme activity at 0.3-0.5 mM
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mRNA + H2O
Saccharomyces cerevisiae
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
yeast, strain W303alpha
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
final specific activity of approximately 1500000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 40°C from poly(U)
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no catalytic activity observed with tRNA and double stranded substrates, little or no reaction with poly(A), poly(I), poly(G), poly(dG)xpoly(dG-dC), and poly(dG-dC)xpoly(dG-dC)
646296
Saccharomyces cerevisiae
?
-
-
-
-
mRNA + H2O
-
646296
Saccharomyces cerevisiae
?
-
-
-
?
mRNA + H2O
mRNA obtained from in vitro transcription
646296
Saccharomyces cerevisiae
?
-
-
-
?
poly(C) x (dG)12-18 polynucleotide template primer + H2O
-
646296
Saccharomyces cerevisiae
?
-
-
-
?
rRNA + H2O
26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA
646296
Saccharomyces cerevisiae
?
-
-
-
?
synthetic homopolynucleotide + H2O
acts on poly(U) and poly(C) with a higher affinity for the latter
646296
Saccharomyces cerevisiae
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
60
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
-
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
7.5
-
Saccharomyces cerevisiae
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
-
-
7.5
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
optimum enzyme activity at 50-100 mM
Saccharomyces cerevisiae
Mg2+
optimum enzyme activity at 0.3-0.5 mM
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mRNA + H2O
Saccharomyces cerevisiae
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
final specific activity of approximately 1500000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 40°C from poly(U)
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no catalytic activity observed with tRNA and double stranded substrates, little or no reaction with poly(A), poly(I), poly(G), poly(dG)xpoly(dG-dC), and poly(dG-dC)xpoly(dG-dC)
646296
Saccharomyces cerevisiae
?
-
-
-
-
mRNA + H2O
-
646296
Saccharomyces cerevisiae
?
-
-
-
?
mRNA + H2O
mRNA obtained from in vitro transcription
646296
Saccharomyces cerevisiae
?
-
-
-
?
poly(C) x (dG)12-18 polynucleotide template primer + H2O
-
646296
Saccharomyces cerevisiae
?
-
-
-
?
rRNA + H2O
26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA
646296
Saccharomyces cerevisiae
?
-
-
-
?
synthetic homopolynucleotide + H2O
acts on poly(U) and poly(C) with a higher affinity for the latter
646296
Saccharomyces cerevisiae
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
60
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
-
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
7.5
-
Saccharomyces cerevisiae
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
-
-
7.5
Other publictions for EC 3.1.26.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646297
Landre
Human endothelial cells select ...
Homo sapiens
J. Cell. Biochem.
86
540-552
2002
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
1
-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646296
Lalioti
Purification and characterizat ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1342
62-72
1997
-
-
-
-
-
-
-
-
-
2
1
1
-
1
-
-
1
-
-
-
1
-
6
1
-
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
6
1
-
1
-
-
1
1
-
1
-
-
-
-
-
-
646294
Laliloti
-
Further characterization of a ...
Ceratitis capitata
Insect Biochem. Mol. Biol.
22
125-135
1992
-
-
-
-
-
-
2
-
-
4
1
1
-
1
-
-
1
-
-
1
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
4
1
1
-
-
-
1
-
1
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646295
Sideris
Preparation of monoclonal anti ...
Ceratitis capitata
Biochem. Int.
27
25-35
1992
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134482
Sideris
Purification and characterizat ...
Ceratitis capitata
Eur. J. Biochem.
164
309-315
1987
-
-
-
-
-
1
2
-
-
1
1
1
-
1
-
-
1
-
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
1
1
-
-
-
1
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-