Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimum enzyme activity at 50-100 mM | Saccharomyces cerevisiae | |
Mg2+ | optimum enzyme activity at 0.3-0.5 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
mRNA + H2O | Saccharomyces cerevisiae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
yeast, strain W303alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
final specific activity of approximately 1500000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 40°C from poly(U) | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no catalytic activity observed with tRNA and double stranded substrates, little or no reaction with poly(A), poly(I), poly(G), poly(dG)xpoly(dG-dC), and poly(dG-dC)xpoly(dG-dC) | Saccharomyces cerevisiae | ? | - |
? | |
mRNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
mRNA + H2O | mRNA obtained from in vitro transcription | Saccharomyces cerevisiae | ? | - |
? | |
poly(C) x (dG)12-18 polynucleotide template primer + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
rRNA + H2O | 26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA | Saccharomyces cerevisiae | ? | - |
? | |
synthetic homopolynucleotide + H2O | acts on poly(U) and poly(C) with a higher affinity for the latter | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
7.5 |