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Literature summary for 3.1.26.10 extracted from

  • Laliloti, V.S.; Sideris, D.C.; Fragoulis, E.G.
    Further characterization of a poly(U), poly(C) ribonuclease from the insect Ceratitis capitata (1992), Insect Biochem. Mol. Biol., 22, 125-135.
No PubMed abstract available
Search for more literature for 3.1.26.10

Inhibitors

Inhibitors Comment Organism Structure
Al3+ strong inhibition Ceratitis capitata
Zn2+ strong inhibition Ceratitis capitata

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ strong stimulation of enzyme activity Ceratitis capitata
Ca2+ strong stimulation of enzyme activity Ceratitis capitata
Cs+ rather weak stimulation Ceratitis capitata
Mn2+ strong stimulation of enzyme activity Ceratitis capitata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Ceratitis capitata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mRNA + H2O Ceratitis capitata translational control ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ceratitis capitata
-
 homometabolous insect
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ceratitis capitata

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Ceratitis capitata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 final specific activity of approximately 1500000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 30°C from poly(U) Ceratitis capitata
additional information
-
 storage at 80°C does not modify the specific activity of the purified protein Ceratitis capitata

Storage Stability

Storage Stability Organism
-80°C, storage does not modify the specific activity of the purified protein Ceratitis capitata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mRNA + H2O translational control Ceratitis capitata ?
-
 ?
poly(C) x (dG)12-18 polynucleotide template primer + H2O due to the presence of the poly(C) tail Ceratitis capitata ?
-
 ?
synthetic homopolynucleotide + H2O acts on poly(U) and poly(C) with a higher affinity for the latter Ceratitis capitata ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Ceratitis capitata