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show all sequences of 3.1.26.10

Purification and characterization of a ribonuclease specific for poly(U) and poly(C) from the larvae of Ceratitis capitata

Sideris, D.C.; Fragoulis, E.G.; Eur. J. Biochem. 164, 309-315 (1987)

Data extracted from this reference:

General Stability
General Stability
Organism
very unstable, DTT, GSH or 2-mercaptoethanol stabilizes
Ceratitis capitata
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
1 mM reduces activity by 15%
Ceratitis capitata
additional information
not inhibited by the placental RNase inhibitor
Ceratitis capitata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
dependent on the concentration, optimum activity at 30-50 mM
Ceratitis capitata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
Ceratitis capitata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mRNA + H2O
Ceratitis capitata
control of protein synthesis in eucaryotes
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ceratitis capitata
-
homometabolous insect
-
Purification (Commentary)
Commentary
Organism
-
Ceratitis capitata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
larva
-
Ceratitis capitata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 1000000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 30°C under standard assay conditions
Ceratitis capitata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
specifically degrades poly(U) and poly(C), fails to degrade other single-stranded homopolyribonucleotides
134482
Ceratitis capitata
?
-
-
-
-
mRNA + H2O
control of protein synthesis in eucaryotes
134482
Ceratitis capitata
?
-
-
-
?
RNA + H2O
RNA isolated from six-day-old larvae or rat liver
134482
Ceratitis capitata
?
-
-
-
?
synthetic homopolynucleotide + H2O
acts on poly(U) and poly(C) with a higher affinity for the latter
134482
Ceratitis capitata
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Ceratitis capitata
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
65
thermolabile, practically loses most of its activity after 10 min at 60°C, at 65°C enzyme is almost inactivated within the first min, at 99°C it is completely inactive right from the beginning
Ceratitis capitata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
Tris-HCl buffer
Ceratitis capitata
General Stability (protein specific)
General Stability
Organism
very unstable, DTT, GSH or 2-mercaptoethanol stabilizes
Ceratitis capitata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
1 mM reduces activity by 15%
Ceratitis capitata
additional information
not inhibited by the placental RNase inhibitor
Ceratitis capitata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
dependent on the concentration, optimum activity at 30-50 mM
Ceratitis capitata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
gel filtration
Ceratitis capitata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mRNA + H2O
Ceratitis capitata
control of protein synthesis in eucaryotes
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ceratitis capitata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
larva
-
Ceratitis capitata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activity 1000000 U/mg, 1 unit is the amount of enzyme producing 0.1 A260 acid-soluble material in 30 min at 30°C under standard assay conditions
Ceratitis capitata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
specifically degrades poly(U) and poly(C), fails to degrade other single-stranded homopolyribonucleotides
134482
Ceratitis capitata
?
-
-
-
-
mRNA + H2O
control of protein synthesis in eucaryotes
134482
Ceratitis capitata
?
-
-
-
?
RNA + H2O
RNA isolated from six-day-old larvae or rat liver
134482
Ceratitis capitata
?
-
-
-
?
synthetic homopolynucleotide + H2O
acts on poly(U) and poly(C) with a higher affinity for the latter
134482
Ceratitis capitata
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Ceratitis capitata
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
65
thermolabile, practically loses most of its activity after 10 min at 60°C, at 65°C enzyme is almost inactivated within the first min, at 99°C it is completely inactive right from the beginning
Ceratitis capitata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
Tris-HCl buffer
Ceratitis capitata
Other publictions for EC 3.1.26.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646297
Landre
Human endothelial cells select ...
Homo sapiens
J. Cell. Biochem.
86
540-552
2002
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-
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2
-
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-
1
-
-
2
-
-
1
-
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
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2
-
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-
1
-
-
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-
1
-
9
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646296
Lalioti
Purification and characterizat ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1342
62-72
1997
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-
-
-
-
-
-
-
-
2
1
1
-
1
-
-
1
-
-
-
1
-
6
1
-
1
-
-
1
1
-
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
6
1
-
1
-
-
1
1
-
1
-
-
-
-
-
-
646294
Laliloti
-
Further characterization of a ...
Ceratitis capitata
Insect Biochem. Mol. Biol.
22
125-135
1992
-
-
-
-
-
-
2
-
-
4
1
1
-
1
-
-
1
-
-
1
1
1
3
1
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
2
-
-
-
4
1
1
-
-
-
1
-
1
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646295
Sideris
Preparation of monoclonal anti ...
Ceratitis capitata
Biochem. Int.
27
25-35
1992
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-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
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-
1
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-
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-
-
-
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-
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-
-
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-
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-
-
-
-
-
-
-
1
-
1
-
-
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-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134482
Sideris
Purification and characterizat ...
Ceratitis capitata
Eur. J. Biochem.
164
309-315
1987
-
-
-
-
-
1
2
-
-
1
1
1
-
1
-
-
1
-
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
1
1
-
-
-
1
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
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-
-