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Literature summary for 3.1.26.1 extracted from

  • Pilly, D.; Niemeyer, A.; Schmidt, M.; Bargetzi, J.P.
    Enzymes for RNA sequence analysis. Preparation and specificity of exoplasmodial ribonucleases I and II from Physarum polycephalum (1978), J. Biol. Chem., 253, 437-445.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion, both enzyme forms Physarum polycephalum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
RNase Phy I, gel filtration Physarum polycephalum
24500
-
RNase Phy II, gel filtration Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
2 enzyme forms: Rnase Phy I and RNase Phy II
-

Purification (Commentary)

Purification (Comment) Organism
RNase Phy I and II Physarum polycephalum

Source Tissue

Source Tissue Comment Organism Textmining
additional information submersed culture Physarum polycephalum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of RNase Phy I and II, the latter shows a preference for GpN substrates, while rejecting ApN dinucleotide substrates, RNase I shows a preference for ApN before GpN substrate, while CpN dinucleotides are rather resistant against hydrolysis, overview Physarum polycephalum ?
-
?
CpC + H2O
-
Physarum polycephalum 5'-CMP + cytidine
-
?
ApG + H2O
-
Physarum polycephalum 5'-GMP + adenosine
-
?
ApA + H2O
-
Physarum polycephalum 5'-AMP + adenosine
-
?
ApC + H2O
-
Physarum polycephalum 5'-CMP + adenosine
-
?
CpU + H2O
-
Physarum polycephalum 5'-UMP + cytidine
-
?
ApU + H2O
-
Physarum polycephalum 5'-UMP + adenosine
-
?
CpA + H2O
-
Physarum polycephalum 5'-AMP + cytidine
-
?
CpG + H2O
-
Physarum polycephalum 5'-GMP + cytidine
-
?
GpA + H2O
-
Physarum polycephalum 5'-AMP + guanosine
-
?
GpC + H2O
-
Physarum polycephalum 5'-CMP + guanosine
-
?
GpG + H2O
-
Physarum polycephalum 5'-GMP + guanosine
-
?
GpU + H2O
-
Physarum polycephalum 5'-UMP + guanosine
-
?
RNA + H2O low molecular weigt yeast RNA Physarum polycephalum 5'-phosphonucleotide product identification ?
UpA + H2O
-
Physarum polycephalum 5'-AMP + uridine
-
?
UpC + H2O
-
Physarum polycephalum 5'-CMP + uridine
-
?
UpG + H2O
-
Physarum polycephalum 5'-GMP + uridine
-
?
UpU + H2O
-
Physarum polycephalum 5'-UMP + uridine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
RNase Phy I and II, assay at Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
RNase Phy II, assay at Physarum polycephalum
3.1
-
RNase Phy II, assay at Physarum polycephalum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
nactivation, RNase Phy I and II Physarum polycephalum

pI Value

Organism Comment pI Value Maximum pI Value
Physarum polycephalum RNase Phy I, isoelectric focusing
-
4.2
Physarum polycephalum RNase Phy II, isoelectric focusing
-
4.2