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Literature summary for 3.1.26.1 extracted from

  • Inokuchi, N.; Saitoh, S.; Kobayashi, H.; Itagaki, T.; Koyama, T.; Uchiyama, S.; Irie, M.
    Comparison of base specificity and other enzymic properties of two protozoan ribonucleases from Physarum polycephalum and Dictyostelium discoideum (1999), Biosci. Biotechnol. Biochem., 63, 141-145.
    View publication on PubMed
Search for more literature for 3.1.26.1

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage to a 5'-phosphomonoester B1 site, i.e. base recognition site for the base at 5'-side of scissile phosphodiester bond, and B2 site, i.e. base recognition site for the base at 3'-side of the scissile bond, identification Physarum polycephalum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ApA + H2O
-
 Physarum polycephalum 5'-AMP + adenosine
-
 ?
ApC + H2O
-
 Physarum polycephalum 5'-CMP + adenosine
-
 ?
ApG + H2O
-
 Physarum polycephalum 5'-GMP + adenosine
-
 ?
ApU + H2O
-
 Physarum polycephalum 5'-UMP + adenosine
-
 ?
CpA + H2O
-
 Physarum polycephalum 5'-AMP + cytidine
-
 ?
CpC + H2O
-
 Physarum polycephalum 5'-CMP + cytidine
-
 ?
CpG + H2O
-
 Physarum polycephalum 5'-GMP + cytidine
-
 ?
CpU + H2O
-
 Physarum polycephalum 5'-UMP + cytidine
-
 ?
GpA + H2O
-
 Physarum polycephalum 5'-AMP + guanosine
-
 ?
GpC + H2O
-
 Physarum polycephalum 5'-CMP + guanosine
-
 ?
GpG + H2O
-
 Physarum polycephalum 5'-GMP + guanosine
-
 ?
GpU + H2O
-
 Physarum polycephalum 5'-UMP + guanosine
-
 ?
additional information substrate specificity Physarum polycephalum ?
-
 ?
poly(I) + H2O
-
 Physarum polycephalum 5'-IMP
-
 ?
polyA + H2O
-
 Physarum polycephalum AMP
-
 ?
polyC + H2O
-
 Physarum polycephalum 5'CMP
-
 ?
polyU + H2O
-
 Physarum polycephalum 5'UMP
-
 ?
RNA + H2O yeast RNA, base specificity of B1 site is A,G,U > C and of B2 A > G > C > U, depolymerization Physarum polycephalum mononucleotides
-
 ?
UpA + H2O
-
 Physarum polycephalum 5'-AMP + uridine
-
 ?
UpC + H2O
-
 Physarum polycephalum 5'-CMP + uridine
-
 ?
UpG + H2O
-
 Physarum polycephalum 5'-GMP + uridine
-
 ?
UpU + H2O
-
 Physarum polycephalum 5'-UMP + uridine
-
 ?

Synonyms

Synonyms Comment Organism
RNase Phyb
-
 Physarum polycephalum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Physarum polycephalum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 stable upto 45°C, loss of approximately 30% activity at 50°C, inactive at 55°C Physarum polycephalum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Physarum polycephalum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 very labile below pH 3.0, stable up to pH 6.0 Physarum polycephalum