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Literature summary for 3.1.25.1 extracted from

  • Jiang, Y.; Rabbi, M.; Kim, M.; Ke, C.; Lee, W.; Clark, R.L.; Mieczkowski, P.A.; Marszalek, P.E.
    UVA generates pyrimidine dimers in DNA directly (2009), Biophys. J., 96, 1151-1158.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.25.1

Application

Application Comment Organism
molecular biology the enzyme is used as a gold standard for detection and/or calibration of DNA lesions (T4 endonuclease V-sensitive sites) caused by UVA damage Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli enzyme detects apurinic/apyrimidinic site base paired with adenine ?
-
 ?
additional information Escherichia coli enzyme detects cyclobutane pyrimidine dimers (DNA lesions) caused by UVA irradiation ?
-
 ?
additional information Escherichia coli UVA light directly generates a significant number of cyclobutane pyrimidine dimers as detected by a combined action of endonuclease IV and T4 endonuclease V ?
-
 ?
supercoiled DNA + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 T4 endonuclease V has both DNA glycosylase and AP-lyase activity, which means that it has the same endonucleolytic activity as Escherichia coli endonuclease IV to cleave the phosphodiester bond at apurinic/apyrimidinic sites Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme detects apurinic/apyrimidinic site base paired with adenine Escherichia coli ?
-
 ?
additional information enzyme detects cyclobutane pyrimidine dimers (DNA lesions) caused by UVA irradiation Escherichia coli ?
-
 ?
additional information UVA light directly generates a significant number of cyclobutane pyrimidine dimers as detected by a combined action of endonuclease IV and T4 endonuclease V Escherichia coli ?
-
 ?
supercoiled DNA + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
T4 endonuclease V
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli