Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Tequatrovirus T4 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme and mutants in complex with DNA containing a thymidine-dimer, X-ray diffraction structure determination and analysis at 1.45-1.6 A and 2.75 A resolution, the latter by molecular replacement method | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
E23D | active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme | Tequatrovirus T4 |
E23Q | active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme | Tequatrovirus T4 |
R3Q | active site mutant, retains full substrate binding ability but shows complex structure differences, crystal structure determination with a bound DNA duplex in comparison to the wild-type enzyme | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | first reaction step of the repair pathway to excise pyrimidine-dimers | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | damaged DNA substrate recognition mechanism, 2 distinct catalytic activities: cleavage of the glycosyl bond at the 5'side of pyrimidine dimer and subsequent scission of the phosphodiester bond at the 3' position of an abasic site through beta-elimination, the enzyme also scans nontarget sequences to search for lesions in DNA duplexes, glycosylase active site involves the catalytic residues Arg3, Glu23, Arg22, and Arg26 and the amino terminus which forms an imino-covalent intermediate with C1' of the 5' side of the thymidine-dimer | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | first reaction step of the repair pathway to excise pyrimidine-dimers | Tequatrovirus T4 | ? | - |
? | |
DNA + H2O | excision of thymine dimers produced within duplex DNA by UV radiation | Tequatrovirus T4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 2 enzyme activities are located in one compact domain consisting of 3 alpha helices and a connecting loop | Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
bacteriophage T4 endodeoxyribonuclease V | - |
Tequatrovirus T4 |
endodeoxyribonuclease (pyrimidine dimer) | - |
Tequatrovirus T4 |
T4 Endo V | - |
Tequatrovirus T4 |
T4 endonuclease V | - |
Tequatrovirus T4 |