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Literature summary for 3.1.25.1 extracted from
- Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA (1981), Proc. Natl. Acad. Sci. USA, 78, 2742-2746.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | - |
Tequatrovirus T4 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
SDS-PAGE, gel filtration | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Tequatrovirus T4 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | cleavage on the 5'-side of dimers | Tequatrovirus T4 | |
| endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| additional information | not: DNA damaged by 4-nitroquinoline-N-oxide or methyl methanesulfonate | Tequatrovirus T4 | ? | - |
? | |
| poly(dA)-poly(dT) + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? | |
| T4 DNA | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
glycosylase activity decreases linearly: loss of 4% of activity per min | Tequatrovirus T4 |
| 42 | - |
endonuclease activity decreases by 50% after 3 min, after 20 min ca. 25% of activity remains | Tequatrovirus T4 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
| 6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
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Release 2023.1 (January 2023)
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