Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
Y129N | dimer-specific binding and nicking abolished, apurinic-specific nicking abolished | Tequatrovirus T4 |
Y131N | in combination with Y129N, dimer-specific binding and nicking abolished, ability for apurinic-specific nicking retained | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
native DNA + H2O | E. coli DNA in vivo | Tequatrovirus T4 | oligonucleotides | - |
? | |
pBR322 DNA + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | - |
? |