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Literature summary for 3.1.22.1 extracted from

  • Anai, M.; Muta, A.; Umeno, M.; Sasaki, M.
    Purification and properties of an acid deoxyribonuclease from rat small intestinal mucosa (1983), J. Biochem., 94, 339-344.
    View publication on PubMed
Search for more literature for 3.1.22.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 50% activation at 20 mM Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol little effect on activity at 10 mM Rattus sp.
Co2+ inhibition above 10 mM Rattus sp.
iodoacetate 43% inhibition at 1 mM, 93% inhibition at 5 mM Rattus sp.
Mg2+ inhibition above 10 mM Rattus sp.
Mn2+ inhibition above 10 mM Rattus sp.
N-ethylmaleimide little effect on activity at 10 mM Rattus sp.
SO42- slightly inhibitory Rattus sp.
tRNA from E. coli, little effect on activity Rattus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ more than 50% activation at 10 mM Rattus sp.
Co2+ slight activation at 10 mM, inhibition at higher concentrations Rattus sp.
Mg2+ slight activation at 10 mM, inhibition at higher concentrations Rattus sp.
Mn2+ slight activation at 10 mM, inhibition at higher concentrations Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 sucrose density gradient centrifugation Rattus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
polyethyleneglycol activity remaines unchanged when stored at -40°C for 12 months Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity by chromatography steps and isoelectric focusing Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
-
 Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O native DNA degraded 2.5 times faster than heat-denatured DNA Rattus sp. 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides oligonucleotides with an average chain length of about seven ?
DNA + H2O
-
 Rattus sp. oligonucleotides + ?
-
 ?
supercoiled plasmid DNA + H2O
-
 Rattus sp. linearized plasmid DNA + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2 4.7
-
 Rattus sp.