Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Thermococcus barophilus | |
Co2+ | - |
Thermococcus barophilus | |
NaCl | activity is suppressed by high NaCl concentration. When adding 500 mM NaCl in the cleavage reaction, the cleavage efficiency of the enzyme is reduced to be approximately 50%. At NaCl concentrations over 800 mM, only slight endonuclease activity remains | Thermococcus barophilus | |
Zn2+ | - |
Thermococcus barophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | a divalent metal ion is required for the enzyme to cleave DNA, Mn2+ (1 mM) and Mg2+ (1 mM) are optimal | Thermococcus barophilus | |
Mn2+ | a divalent metal ion is required for the enzyme to cleave DNA, Mn2+ (1 mM) and Mg2+ (1 mM) are optimal | Thermococcus barophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Thermococcus barophilus | endonuclease V is an important enzyme for repairing deoxyinosine in DNA | ? | - |
? | |
DNA + H2O | Thermococcus barophilus Ch5 | endonuclease V is an important enzyme for repairing deoxyinosine in DNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus barophilus | A0A0S1X9V3 | - |
- |
Thermococcus barophilus Ch5 | A0A0S1X9V3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | endonuclease V is an important enzyme for repairing deoxyinosine in DNA | Thermococcus barophilus | ? | - |
? | |
DNA + H2O | the enzyme exhibits a higher affinity for binding to deoxyinosine-containing DNA than normal DNA | Thermococcus barophilus | ? | - |
? | |
DNA + H2O | endonuclease V is an important enzyme for repairing deoxyinosine in DNA | Thermococcus barophilus Ch5 | ? | - |
? | |
DNA + H2O | the enzyme exhibits a higher affinity for binding to deoxyinosine-containing DNA than normal DNA | Thermococcus barophilus Ch5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo V | - |
Thermococcus barophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | - |
Thermococcus barophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme withstands 100°C for 120 min without significant loss of its activity | Thermococcus barophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Thermococcus barophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | between pH 6.0 and pH 9.0, the enzyme exhibits more than 86% cleavage efficiency | Thermococcus barophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | endonuclease V is an important enzyme for repairing deoxyinosine in DNA | Thermococcus barophilus |