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Literature summary for 3.1.21.7 extracted from

  • Kiyonari, S.; Egashira, Y.; Ishino, S.; Ishino, Y.
    Biochemical characterization of endonuclease V from the hyperthermophilic archaeon, Pyrococcus furiosus (2014), J. Biochem., 155, 325-333.
    View publication on PubMed
Search for more literature for 3.1.21.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
D35A inactive mutant enzyme Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
NaCl 200 mM, complete inhibition Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ Pyrococcus furiosus
Mn2+ activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Pyrococcus furiosus endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand ?
-
 ?
nicked DNA + H2O Pyrococcus furiosus
-
 ?
-
 ?
RNA + H2O Pyrococcus furiosus the enzyme also cleaves inosine-containing RNA strands ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U263
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand Pyrococcus furiosus ?
-
 ?
dsDNA containing deoxyinosine + H2O no cleavage activity is detected with the dsDNA containing a T/G mismatch base pair and a dsDNA containing an apurinic site. The enzyme possesses strict substrate specificity to deoxyinosine-containing DNA. The enzyme cleaves inosine-containing RNA strands as well as DNA substrates Pyrococcus furiosus ?
-
 ?
nicked DNA + H2O
-
 Pyrococcus furiosus ?
-
 ?
RNA + H2O the enzyme also cleaves inosine-containing RNA strands Pyrococcus furiosus ?
-
 ?

Synonyms

Synonyms Comment Organism
endo V
-
 Pyrococcus furiosus
endonuclease V
-
 Pyrococcus furiosus
EndoV
-
 Pyrococcus furiosus
PF0987 locus name Pyrococcus furiosus
PfuEndoV
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 a moderate reaction temperature is used for the biochemical studies, because of the melting temperature of the DNA duplex substrates (Tm = 67ºC) Pyrococcus furiosus
90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 60°C: about 45% of maximal activity, 100°C: about 70% of maximal activity Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.8 pH 7.0: about 45% of maximal activity, pH 8.8: about 90% of maximal activity Pyrococcus furiosus

General Information

General Information Comment Organism
metabolism this DNA repair enzyme is also involved in RNA metabolism Pyrococcus furiosus
physiological function DNA repair enzyme, may also be involved in RNA metabolism Pyrococcus furiosus