Literature summary for 3.1.21.7 extracted from

Feng, H.; Dong, L.; Cao, W.
Catalytic Mechanism of Endonuclease V: A Catalytic and Regulatory Two-Metal Model (2006), Biochemistry, 45, 10251-10259.

Search for more literature for 3.1.21.7

Protein Variants

Protein Variants	Comment	Organism
D110A	little cleavage activity	Thermotoga maritima
D110C	mutant	Thermotoga maritima
D110E	mutant	Thermotoga maritima
D110H	mutant	Thermotoga maritima
D43A	no more than 10% cleavage activity	Thermotoga maritima
D43C	no more than 10% cleavage activity	Thermotoga maritima
D43E	no more than 10% cleavage activity	Thermotoga maritima
D43H	no more than 10% cleavage activity	Thermotoga maritima
E89A	about 20-30% cleavage activity	Thermotoga maritima
E89D	mutant	Thermotoga maritima
E89H	mutant	Thermotoga maritima
H214A	tolerant of mutation without impacting activity	Thermotoga maritima
H214C	tolerant of mutation without impacting activity	Thermotoga maritima
H214D	little change in substrate specificity or DNA cleavage kinetics	Thermotoga maritima
H214D	tolerant of mutation without impacting activity	Thermotoga maritima
H214E	tolerant of mutation without impacting activity	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	no catalytic function, supports binding to DNA	Thermotoga maritima
Mg2+	-	Thermotoga maritima
Mn2+	in the presence of Mn2+, 3'-exonuclease activity is pronounced in wild type and mutant enzymes E89D, D110C, H214C, H214E, and H214D, while reduced activity is detected in D110A, D110H, and D110E	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X2H9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	hydrolysis of the second phosphodiester bond 3' from a deaminated base lesion including inosine, xanthosine, oxanosine and uridine	Thermotoga maritima	hydrolysed DNA	-	?

Synonyms

Synonyms	Comment	Organism
endonuclease V	-	Thermotoga maritima

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Release 2023.1 (January 2023)