Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.21.7 extracted from

  • Gates III, F.T.; Linn, S.
    Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays (1977), J. Biol. Chem., 252, 2802-2807.
    View publication on PubMed
Search for more literature for 3.1.21.7

Inhibitors

Inhibitors Comment Organism Structure
NaCl complete inhibition at 1 M Escherichia coli
tRNA half-maximal inhibition rate at 0.08 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 glycerol density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli also nicks duplex DNA exposed to OsO4, x-rays or acid, does not act upon undamaged DNA or irradiated single-stranded DNA 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O also nicks duplex DNA exposed to OsO4, x-rays or acid, does not act upon undamaged DNA or irradiated single-stranded DNA Escherichia coli 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 50% remaining activity Escherichia coli