Application | Comment | Organism |
---|---|---|
additional information | deoxyribonuclease is an endonuclease, but is not a restriction endonuclease | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | chelates Mg2+, increasing Mg2+ concentrations can restore the activity of the enzyme | Mycolicibacterium smegmatis | |
K+ | - |
Mycolicibacterium smegmatis | |
additional information | not inhibited by Na+ | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, maximum activity at 2.5 mM | Mycolicibacterium smegmatis | |
Mn2+ | can replace Mg2+ showing better activity | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
mc26 culture | - |
Purification (Comment) | Organism |
---|---|
to near homogeneity, 521fold by gel filtration | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
crude extract | Mycolicibacterium smegmatis |
9444 | - |
521fold purified enzyme | Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, stable for at least 15 days | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
covalently closed circular DNA pUC18 + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
lambda DNA + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
additional information | catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically | Mycolicibacterium smegmatis | ? | - |
? | |
pBR322 DNA + H2O | - |
Mycolicibacterium smegmatis | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Deoxyriboendonuclease | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Mycolicibacterium smegmatis |