Literature summary for 3.1.21.6 extracted from

  • Mandal, P.; Chakraborty, P.; Sau, S.; Mandal, N.C.
    Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis (2006), J. Biochem. Mol. Biol., 39, 140-144.
    View publication on PubMed

Application

Application Comment Organism
additional information deoxyribonuclease is an endonuclease, but is not a restriction endonuclease Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
EDTA chelates Mg2+, increasing Mg2+ concentrations can restore the activity of the enzyme Mycolicibacterium smegmatis
K+
-
Mycolicibacterium smegmatis
additional information not inhibited by Na+ Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ can replace Mg2+ showing better activity Mycolicibacterium smegmatis
Mg2+ required for activity, maximum activity at 2.5 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
mc26 culture
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity, 521fold by gel filtration Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
crude extract Mycolicibacterium smegmatis
9444
-
521fold purified enzyme Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, stable for at least 15 days Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically Mycolicibacterium smegmatis ?
-
?
covalently closed circular DNA pUC18 + H2O
-
Mycolicibacterium smegmatis ?
-
?
lambda DNA + H2O
-
Mycolicibacterium smegmatis ?
-
?
pBR322 DNA + H2O
-
Mycolicibacterium smegmatis DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively
-
?

Synonyms

Synonyms Comment Organism
Deoxyriboendonuclease
-
Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Mycolicibacterium smegmatis