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Literature summary for 3.1.21.5 extracted from

  • Sears, A.; Szczelkun, M.D.
    Subunit assembly modulates the activities of the Type III restriction-modification enzyme PstII in vitro (2005), Nucleic Acids Res., 33, 4788-4796.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Providencia stuartii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Providencia stuartii the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O Providencia stuartii 164 the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Providencia stuartii
-
-
-
Providencia stuartii 164
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Providencia stuartii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Providencia stuartii specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo Providencia stuartii specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O recognition sequence: CTGATG. The enzyme requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of ATP hydrolysis (about 40 ATP/site/min). PstII can cut DNA with GTP and CTP Providencia stuartii specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O
-
 Providencia stuartii 164 specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo Providencia stuartii 164 specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O recognition sequence: CTGATG. The enzyme requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of ATP hydrolysis (about 40 ATP/site/min). PstII can cut DNA with GTP and CTP Providencia stuartii 164 specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo Providencia stuartii

Synonyms

Synonyms Comment Organism
PstII
-
 Providencia stuartii
restriction endonuclease PstII
-
 Providencia stuartii