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Literature summary for 3.1.21.5 extracted from

  • Möncke-Buchner, E.; Mackeldanz, P.; Kruger, D.H.; Reuter, M.
    Overexpression and affinity chromatography purification of the Type III restriction endonuclease EcoP15I for use in transcriptome analysis (2004), J. Biotechnol., 114, 99-106.
    View publication on PubMed

Application

Application Comment Organism
analysis transcriptome analysis Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pQE-16 plasmid vector that provides the enzyme with a C-terminal 6His-tag Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O ECoP15I recognizes the non-symmetric DNA sequence 5'-CAGCAG. For efficient cleavage, the enzyme needs the interaction with two copies of the recognition sequence that have to be inversely oriented in the DNA double-strand. The enzyme cuts the upper DNA strand 25-26 bp and the lower DNA strand 27-28 bp, respectively, downstream of the recognition sequence Escherichia coli specific double-stranded DNA fragments with terminal 5'-phosphate
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Synonyms

Synonyms Comment Organism
EcoP15I
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Escherichia coli