Application | Comment | Organism |
---|---|---|
analysis | transcriptome analysis | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
cloned into pQE-16 plasmid vector that provides the enzyme with a C-terminal 6His-tag | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | ECoP15I recognizes the non-symmetric DNA sequence 5'-CAGCAG. For efficient cleavage, the enzyme needs the interaction with two copies of the recognition sequence that have to be inversely oriented in the DNA double-strand. The enzyme cuts the upper DNA strand 25-26 bp and the lower DNA strand 27-28 bp, respectively, downstream of the recognition sequence | Escherichia coli | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EcoP15I | - |
Escherichia coli |