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Literature summary for 3.1.21.4 extracted from

  • Zhang, Z.; Xu, K.; Xin, Y.; Zhang, Z.
    An efficient method for multiple site-directed mutagenesis using type IIs restriction enzymes (2015), Anal. Biochem., 476, 26-28 .
    View publication on PubMed

Application

Application Comment Organism
molecular biology site-directed mutagenesis methods are very important in modern molecular biology, biochemistry, and protein engineering. A site-directed mutagenesis method that can be used for multiple mutation generation using type IIs restriction enzymes. This approach is faster and more convenient than the overlap polymerase chain reaction method due to its having fewer reaction steps and being cheaper than, but as convenient as, enzymatic assembly Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q69B20
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Synonyms

Synonyms Comment Organism
BsmBI
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Geobacillus stearothermophilus