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Literature summary for 3.1.21.4 extracted from
- Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency (2012), Nucleic Acids Res., 40, e149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Citrobacter freundii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D134N/K190N/E204V | inactive | Citrobacter freundii |
| E204Q | the mutant possesses 0.004% of wild type activity | Citrobacter freundii |
| E71A/K190T/E204I | inactive | Citrobacter freundii |
| E71A/K190T/E204V | inactive | Citrobacter freundii |
| E71A/S188A/K190T/E204Q | inactive | Citrobacter freundii |
| E71N/D254G | inactive | Citrobacter freundii |
| E71Q | the mutant possesses 10% of wild type activity | Citrobacter freundii |
| S188A | the mutant possesses 4% of wild type activity | Citrobacter freundii |
| S188C | the mutant possesses 0.5% of wild type activity | Citrobacter freundii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Citrobacter freundii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Citrobacter freundii | the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star | Citrobacter freundii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| phage lambda DNA + H2O | - |
Citrobacter freundii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Citrobacter freundii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cfr10I | - |
Citrobacter freundii |
| type II REase | - |
Citrobacter freundii |
| type II restriction endonuclease | - |
Citrobacter freundii |
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Release 2023.1 (January 2023)
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