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Literature summary for 3.1.21.4 extracted from
- Distinct facilitated diffusion mechanisms by E. coli Type II restriction endonucleases (2014), Biochemistry, 53, 7028-7037.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Escherichia coli | EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| additional information | Escherichia coli | EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | EcoRI recognizes 5'-GAATTC-3' while EcoRV recognizes 5'-GATATC-3', leaving overhangs and blunt DNA segments, respectively | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| additional information | EcoRV utilizes intersegmental hopping to a greater extent than does EcoRI | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EcoRI | - |
Escherichia coli |
| EcoRV | - |
Escherichia coli |
| type II ENase | - |
Escherichia coli |
| type II restriction enonuclease | - |
Escherichia coli |
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