Application | Comment | Organism |
---|---|---|
molecular biology | tools for the dissection, analysis and reconstruction of DNA | Pyrococcus abyssi |
Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimally active at NaCl concentrations ranging from 100-200 mM | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | G8ZFZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | the enzyme recognizes 5'-GTAC and leaves a 3'-TA overhang (5'-GTA/C) | Pyrococcus abyssi | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PabI | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
1 h, the enzyme retains approximately 50% of its activity | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus abyssi |