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Literature summary for 3.1.21.4 extracted from

  • Guan, S.; Blanchard, A.; Zhang, P.; Zhu, Z.
    Alteration of sequence specificity of the type IIS restriction endonuclease Btsi (2010), PLoS ONE, 5, e11787.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.4

Protein Variants

Protein Variants Comment Organism
R119A subunit BtsIB mutant shows a different digestion pattern from the wild type BtsI. The mutant BtsIB(R119A) acts as a different restriction enzyme with a previously unreported recognition sequence CAGTG(2/0), which is named as BtsI-1 Parageobacillus thermoglucosidasius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Parageobacillus thermoglucosidasius BtsI recognizes and digests at GCAGTG(2/0) ?
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 ?

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information BtsI recognizes and digests at GCAGTG(2/0) Parageobacillus thermoglucosidasius ?
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 ?

Subunits

Subunits Comment Organism
dimer subunits, BtsIA and BtsIB. The BtsIB subunit contains the recognition domain, one catalytic domain for bottom strand nicking and part of the catalytic domain for the top strand nicking. BtsIA has the rest of the catalytic domain that is responsible for the DNA top strand nicking. BtsIA alone has no activity unless it mixes with BtsIB to reconstitute the BtsI activity Parageobacillus thermoglucosidasius

Synonyms

Synonyms Comment Organism
BtsI
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 Parageobacillus thermoglucosidasius
Type IIS REase
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 Parageobacillus thermoglucosidasius
type IIS restriction endonuclease
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 Parageobacillus thermoglucosidasius

General Information

General Information Comment Organism
additional information subunit BtsIB mutant shows a different digestion pattern from the wild type BtsI. The mutant BtsIB(R119A) acts as a different restriction enzyme with a previously unreported recognition sequence CAGTG(2/0), which is named as BtsI-1. Compared with wild-type BtsI, BtsI-1 shows different relative activities in NEB restriction enzyme reaction buffers NEB1, NEB2, NEB3 and NEB4 and less star activity. Similar to the wild-type BtsIB subunit, the BtsI-1 B subunit alone can act as a bottom nicking enzyme recognizing CAGTG(-/0) Parageobacillus thermoglucosidasius