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Literature summary for 3.1.21.4 extracted from
- Domain organization and functional analysis of type IIS restriction endonuclease Eco31I (2008), Biochemistry, 47, 8546-8556.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strains ER2267 and HMS174 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Escherichia coli |
| 67000 | - |
calculated molecular mass of Eco31I | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q8RNY7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, phosphocellulose P-11 column chromatography, heparin-Sepharose column chromatography, AH-Sepharose column chromatography, and Blue-Sepharose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Eco31I | - |
Escherichia coli |
| type II REase | - |
Escherichia coli |
| type IIS restriction endonuclease | - |
Escherichia coli |
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