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Literature summary for 3.1.21.4 extracted from

  • Meramveliotaki, C.; Kotsifaki, D.; Androulaki, M.; Hountas, A.; Eliopoulos, E.; Kokkinidis, M.
    Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease (2007), Acta Crystallogr. Sect. F, 63, 836-838.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Proteus vulgaris

Crystallization (Commentary)

Crystallization (Comment) Organism
single-chain variant scPvuII constructed by tandemly joining the two subunits through the peptide linker Gly-Ser-Gly-Gly. Resolution of 2.35 A, space group P42 Proteus vulgaris

Protein Variants

Protein Variants Comment Organism
additional information conversion of enzyme from its wild-type homodimeric form into the enzymatically active single-chain variant scPvuII by tandemly joining the two subunits through the peptide linker Gly-Ser-Gly-Gly, crystallization data Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
isoform PvuII
-