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Literature summary for 3.1.21.3 extracted from

  • Csefalvay, E.; Lapkouski, M.; Guzanova, A.; Csefalvay, L.; Baikova, T.; Shevelev, I.; Bialevich, V.; Shamayeva, K.; Janscak, P.; Kuta Smatanova, I.; Panjikar, S.; Carey, J.; Weiserova, M.; Ettrich, R.
    Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme (2015), PLoS ONE, 10, e0128700 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
EcoR124I methylase is purified from Escherichia coli strain JM109(DE3) harboring plasmid pJS4M Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion, crystal structures of three mutants of EcoR124I HsdR are designed to probe this mechanism. The results indicate that interdomain engagement via ATP is responsible for signal transmission between the endonuclease and helicase domains of the motor subunit Escherichia coli

Protein Variants

Protein Variants Comment Organism
K220A the mutant enzyme shows a 5fold reduction in restriction activity. The mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex Escherichia coli
K220Q the mutant enzyme shows a 400fold reduction in restriction activity. The mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex Escherichia coli
K220R the mutant motor subunit is not defective in interacting with the methyltransferase to form the endonuclease complex Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P10486 HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124; plasmid R124
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Purification (Commentary)

Purification (Comment) Organism
EcoR124I methylase is purified from Escherichia coli strain JM109(DE3) harboring plasmid pJS4M Escherichia coli

Synonyms

Synonyms Comment Organism
HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124 Escherichia coli