Literature summary for 3.1.21.3 extracted from

Uyen, N.T.; Nishi, K.; Park, S.Y.; Choi, J.W.; Lee, H.J.; Kim, J.S.
Crystallization and preliminary X-ray diffraction analysis of the HsdR subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016 (2008), Acta Crystallogr. Sect. F, 64, 926-928.

Cloned(Commentary)

Cloned (Comment)	Organism
HsdR subunit cloned into pProExHTc and expressed in Escherichia coli B834(DE3)	Vibrio vulnificus YJ016

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop and sitting-drop vapour-diffusion method, HsdR subunit crystallized from 8%(w/v) polyethylene glycol 3350, 0.15 M ammonium chloride, 0.1 M HEPES pH 7.5 and 2 mM beta-mercaptoethanol, to 2.60 A resolution. Crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.01, b = 89.04, c = 113.66 A. With one HsdR molecule in the asymmetric unit, the Matthews coefficient is 2.14 A3 Da-1 and the solvent content is 42%	Vibrio vulnificus YJ016

Crystallization (Comment)

Organism

hanging-drop and sitting-drop vapour-diffusion method, HsdR subunit crystallized from 8%(w/v) polyethylene glycol 3350, 0.15 M ammonium chloride, 0.1 M HEPES pH 7.5 and 2 mM beta-mercaptoethanol, to 2.60 A resolution. Crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.01, b = 89.04, c = 113.66 A. With one HsdR molecule in the asymmetric unit, the Matthews coefficient is 2.14 A3 Da-1 and the solvent content is 42%

Vibrio vulnificus YJ016

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus YJ016	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HsdR fusion protein purified by gel filtration. HsdR with five additional amino acids (YFQGA) at the N-terminus purified on anion-exchange column and by gel filtration, purified protein is more than 95% pure	Vibrio vulnificus YJ016

Synonyms

Synonyms	Comment	Organism
type I restriction enzyme	-	Vibrio vulnificus YJ016

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)