Literature summary for 3.1.21.3 extracted from

Meister, J.; MacWilliams, M.; Hubner, P.; Jutte, H.; Skrzypek, E.; Piekarowicz, A.; Bickle, T.A.
Macroevolution by transposition: drastic modification of DNA recognition by a type I restriction enzyme following Tn5 transposition (1993), EMBO J., 12, 4585-4591.

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Protein Variants

Protein Variants	Comment	Organism
additional information	mutation in which the specificity has altered due to a Tn5 insertion into the middle of the hsdS, the gene which encodes the polypeptide that confers DNA sequence specificity to both the restriction and the modification reaction. The mutant recognition sequence is an interrupted palindrome, TCA(N8)TGA, in which the 5' half site of the wild type site is repeated in inverse orientation. The additional base pair in the non-specific spacer of the mutant recognition sequence maintains the proper spacing between the two methylatable adenine groups. Tn5 insertion occurs at nucleotide 673 of the 1221 bp gene. This effectively deletes the entire carboxyl-terminal DNA binding domain which recognizes the 3' half of the EcoDXXI binding site. The truncated hsdS gene still encodes both the amino-terminal DNA binding domain and the conserved repeated sequence that defines the length of the recognition site spacer region	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	enzyme EcoDXXI	-

Purification (Commentary)

Purification (Comment)	Organism
mutant methylase	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
duplex DNA + ATP	-	Escherichia coli	double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate	-	?

Synonyms

Synonyms	Comment	Organism
EcoDXXI	-	Escherichia coli

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Release 2023.1 (January 2023)