Literature summary for 3.1.21.2 extracted from

Wang, W.W.; Zhou, H.; Xie, J.J.; Yi, G.S.; He, J.H.; Wang, F.P.; Xiao, X.; Liu, X.P.
Thermococcus eurythermalis endonuclease IV can cleave various apurinic/apyrimidinic site analogues in ssDNA and dsDNA (2018), Int. J. Mol. Sci., 20, 69.

Search for more literature for 3.1.21.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km and kcat for cleaving ssDNAs containing dSpacer and Spacer C3, C4, C6, C12, 9, 18 are calculated by double reciprocal plotting using the initial reaction rates at various substrate concentrations	Thermococcus eurythermalis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus eurythermalis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
double-stranded DNA + H2O	the enzyme can cleave various apurinic/apyrimidinic site analogues in ssDNA and dsDNA. The apurinic/apyrimidinic endonuclease activity can incise DNA 5' to various apurinic/apyrimidinic site analogues, including the alkane chain Spacer and polyethylene glycol Spacer. The efficient cleavage at apurinic/apyrimidinic sites requires 2 or more normal nucleotides existing at the 5'-terminus. The enzyme also possesses 3'-exonuclease activity for removing 3'-blocking groups and normal nucleotides	Thermococcus eurythermalis	?	-	?

Synonyms

Synonyms	Comment	Organism
EndoIV	-	Thermococcus eurythermalis
endonuclease IV	-	Thermococcus eurythermalis

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Release 2023.1 (January 2023)