Literature summary for 3.1.21.2 extracted from

Back, J.H.; Chung, J.H.; Park, J.H.; Han, Y.S.
A versatile endonuclease IV from Thermus thermophilus has uracil-excising and 3-5 exonuclease activity (2006), Biochem. Biophys. Res. Commun., 346, 889-895.

Search for more literature for 3.1.21.2

Cloned(Commentary)

Cloned (Comment)	Organism
into the pMal-c2x vector for expression in Escherichia coli TB1 cells	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	in the presence of 10 mM EDTA enzymatic activity is completely eliminated	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Thermus thermophilus
Mn2+	-	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	determined by SDS-PAGE	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72KH8	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a amylose resin affinity column, the fusion protein is cleaved with thrombin to remove the MBP tag, the cleavage mixture is applied to a Q anion-exchange column	Thermus thermophilus

Storage Stability

Storage Stability	Organism
-20°C, Tris-buffer, pH 6.8, 50 mM NaCl, 0.5 mM DTT, 50% glycerol	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
endonuclease IV	endonuclease IV of Thermus thermophilus possesses a 3'-5' exonuclease activity similar to that of Escherichia coli exonuclease III	Thermus thermophilus
TthNfo	-	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay on the uracil-processing activity and 3'-5' exonuclease activity	Thermus thermophilus
7.5	-	DNA cleavage assay	Thermus thermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)