Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required for nicking activity | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded alkylated DNA + H2O | Tequatrovirus T4 | in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks | 5'-phosphooligonucleotides | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
Escherichia coli phage, i.e. Coliphage T4 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products | reaction mechanism modelling | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded alkylated DNA + H2O | in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
ir | |
double-stranded alkylated DNA + H2O | the enzyme cleaves sites of DNA with conserved sequence elements to both the left and the right of the cleaved bonds comprising 16 basepairs with some variability tolerated, nicking of a single and both strands, the latter in two nicking events, substrate specificity, overview, in vitro the enzyme produces mostly single-stranded nicks | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
EndoII | - |
Tequatrovirus T4 |
endonuclease II | - |
Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tequatrovirus T4 |