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Literature summary for 3.1.21.2 extracted from

  • Carlson, K.; Lagerback, P.; Nystrom, A.C.
    Bacteriophage T4 endonuclease II: concerted single-strand nicks yield double-strand cleavage (2004), Mol. Microbiol., 52, 1403-1411.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required for nicking activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded alkylated DNA + H2O Tequatrovirus T4 in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks 5'-phosphooligonucleotides
-
ir

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
Escherichia coli phage, i.e. Coliphage T4
-

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products reaction mechanism modelling Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded alkylated DNA + H2O in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks Tequatrovirus T4 5'-phosphooligonucleotides
-
ir
double-stranded alkylated DNA + H2O the enzyme cleaves sites of DNA with conserved sequence elements to both the left and the right of the cleaved bonds comprising 16 basepairs with some variability tolerated, nicking of a single and both strands, the latter in two nicking events, substrate specificity, overview, in vitro the enzyme produces mostly single-stranded nicks Tequatrovirus T4 5'-phosphooligonucleotides
-
ir

Synonyms

Synonyms Comment Organism
EndoII
-
Tequatrovirus T4
endonuclease II
-
Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Tequatrovirus T4