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Literature summary for 3.1.21.1 extracted from

  • Stefan, A.; Conti, M.; Rubboli, D.; Ravagli, L.; Presta, E.; Hochkoeppler, A.
    Overexpression and purification of the recombinant diphtheria toxin variant CRM197 in Escherichia coli (2010), J. Biotechnol., 156, 245-252.
    View publication on PubMed
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
mutant G52E (CRM197) is expressed in Escherichia coli BL21AI cells Corynebacterium diphtheriae

Protein Variants

Protein Variants Comment Organism
G52E the mutation reduces the diphtheria toxin's toxicity Corynebacterium diphtheriae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Corynebacterium diphtheriae
Mg2+ activates Corynebacterium diphtheriae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Corynebacterium diphtheriae

Organism

Organism UniProt Comment Textmining
Corynebacterium diphtheriae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap chelating column chromatography and Superdex-200 gel filtration Corynebacterium diphtheriae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Corynebacterium diphtheriae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Corynebacterium diphtheriae

Synonyms

Synonyms Comment Organism
CRM197 G52E variant of the diphtheria toxin Corynebacterium diphtheriae
diphtheria toxin
-
 Corynebacterium diphtheriae
DTx
-
 Corynebacterium diphtheriae