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Literature summary for 3.1.21.1 extracted from
- Murine serum nucleases - contrasting effects of plasmin and heparin on the activities of DNase1 and DNase1-like 3 (DNase1/3) (2009), FEBS J., 276, 1059-1073.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Aprotinin | activates DNase1/3-like activity | Mus musculus |  |
| heparin | activates DNase1 | Mus musculus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| NIH-3T3 cells with expression vectors for murine DNase1 and DNase1/3 cDNA | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | DNase1 knockout mice, contain residual nuclease activity. Addition of aprotinin or plasminogen activator inhibitor 1 to the sera of DNase1 KO mice completes chromatolysis to mononucleosomes and even to oligonucleotides | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aprotinin | inhibits DNase1 as a result of plasmin inhibition | Mus musculus | |
| heparin | directly inhibits recombinant DNase1/3 | Homo sapiens | |
| heparin | directly inhibits recombinant DNase1/3 | Mus musculus | |
| additional information | DNase1/3-like nuclease is inhibited by proteolysis of DNA-bound structural proteins but not by thrombin. When serum frozen at -20°C to thawing to room temperature and subsequently stored at 4°C, it looses its DNase1/3-like activity within 2 weeks | Mus musculus | |
| NaCl | increasing concentrations of NaCl (approximately half activity in the presence of 50 mM NaCl) have a greater inhibitory influence on rmDNase1/3 than on rmDNase1 (approximately half activity in the presence of 150 mM NaCl) | Mus musculus | |
| plasmin | directly inhibits recombinant DNase1/3, but does not inactivate recombinant DNase1 | Mus musculus | |
| Tris | increasing concentrations of Tris (approximately half activity in the presence of 80 mM Tris) have a greater inhibitory influence on rmDNase1/3 than on rmDNase1 (no inhibitory influence of Tris) | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
| Mn2+ | activity of recombinant DNase1 in the presence of Mn2+ is increased only slightly, whereas recombinant DNase1/3 displays strongly enhanced nucleolysis | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29800 | - |
calculated molecular mass of DNase1 | Mus musculus |
| 33100 | - |
calculated molecular mass of DNase1/3 | Mus musculus |
| 34000 | - |
DNase1/3, by DPZ | Mus musculus |
| 37000 | - |
DNase1, by DPZ | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | ? | - |
? | |
| additional information | Mus musculus C57BL/6 | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | O55070 | C57BL/6 mice | - |
| Mus musculus C57BL/6 | O55070 | C57BL/6 mice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DNase1/3-like nuclease, by affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | high DNase1 content | Mus musculus | - |
| serum | contains two nucleases, DNase1 and DNase1/3. DNase1/3 is not N-glycosylated, whereas DNase1 is di-N-glycosylated | Mus musculus | - |
| serum | DNase1/3 | Homo sapiens | - |
| spleen | high DNase1/3 content | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chromatin + H2O | recombinant DNase1 degrades chromatin effectively only in cooperation with serine proteases, such as plasmin or thrombin, which remove DNA-bound proteins. Recombinant DNase1/3 degrades chromatin without proteolytic help | Mus musculus | ? | - |
? | |
| chromatin + H2O | recombinant DNase1 degrades chromatin effectively only in cooperation with serine proteases, such as plasmin or thrombin, which remove DNA-bound proteins. Recombinant DNase1/3 degrades chromatin without proteolytic help | Mus musculus C57BL/6 | ? | - |
? | |
| additional information | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | Mus musculus | ? | - |
? | |
| additional information | DNase1 and DNase1/3 may substitute or cooperate with each other during DNA degradation, providing effective clearance after exposure or release from dying cells | Mus musculus C57BL/6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dnase1 | - |
Homo sapiens |
| Dnase1 | - |
Mus musculus |
| DNase1-like 3 | - |
Homo sapiens |
| DNase1-like 3 | - |
Mus musculus |
| DNase1/3 | - |
Homo sapiens |
| DNase1/3 | - |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | pH optimum of recombinant DNase1 is 6.5-7.5 in the presence of Mg2+, and 7.5-8.5 in the presence of Mn2+. PH optimum of recombinant DNase1/3 in the presence of Mg2+ shifts from pH 4.5-5.5 to pH 5.5-6.5 by Mn2+ | Mus musculus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mus musculus | DNase1 | - |
4.9 |
| Mus musculus | DNase1/3 | - |
8.7 |
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